Research Articles

Join WhatsApp Channel 

GPAT caoching
  • PharmaTutor
    19 October 2012

    UV- SPECTROPHOTOMETRIC METHOD DEVELOPMENT FOR THE DETERMENATION OF PARACETAMOL AND DROTAVERINE HYDROCHLORIDE IN COMBINATION TABLET DOSAGE FORM BY SIMULTANEOUS EQUATIONS METHOD

    About Authors:
    Rambabu.CH*, V. V. V. S. P. Apparao, Miss. M.  Muthulakshmi, V. Ananth.
    aPG-Student, Department Of Pharmaceutical Analysis,
    KMCH College of Pharmacy,
    Kalapatti Road, Coimbatore– 641 048, INDIA.
    *ramgepharma@gmail.com

    ABSTRACT:
    At present, simultaneous determination of drugs in the combination dosage forms has been enjoying renaissance in the field of pharmaceutical analysis. Paracetamol, a classical antipyretic in combination with a novel antispasmodic drug, drotaverine hydrochloride provides a synergistic effect in the treatment of spasms. From the reviewed literature, it was simultaneous uv-spectrophotometric methods have not yet been developed for the determination and quantification of paracetamol and drotaverine hydrochloride. The λmax of paracetamol is 257 nm and that of drotaverine hydrochloride were scanned and found to be 308 nm, 352 nm. Both paracetamol and drotaverine hydrochloride were found to have significant absorbance of the λmax   of each other and total absorbance was equal to the sum of the absorbance of paracetamol and drotaverine hydrochloride individually measured. So the present study involves the uv-spectrophotometric method development for the simultaneous determination of paracetamol and drotaverine hydrochloride by using simultaneous equations method. The mean % recoveries from this method were found to be 100.76% and 100.17% for paracetamol and drotaverine hydrochloride respectively proving that the method is accurate.

  • Pharmacological Screening of Ethanolic Extract of Plant Solanum XanthocarpumOn Nephrolithiasis Rats
    17 October 2012
    Pharmacological Screening of Ethanolic Extract of Plant Solanum XanthocarpumOn Nephrolithiasis Rats

    About Authors:
    Amar Patil*, Shraddha Kanase
    M.Pharm (Pharmacology)    
    Department Of Pharmacology, Tatyasaheb Kore College Of Pharmacy,
    Warananagar. Dist;Kolhapur, India .
    *appatil.tkcp@gmail.com

    Abstract:
    Solanum Xanthocarpumhas been known traditionally in Indian system of medicine for the treatment of various metabolic disease and disorders. Since, the present study was carried out to know its applicability in urolithiasis condition. In the present study, extracts of Solanum Xanthocarpumwas prepared with Ethanol and used for evaluation of antiurolithiatic activity. In this study we systematically evaluated its property by using Ethylene Glycol induced Albinorats. This study also involves state of the art animal model to elucidate its probable mechanism of industrial guidelines of FDA, USA for pre-clinical evaluation of anti-Nephrolithiasis drugs.

  • SIMULTANEOUS DETERMINATION OF IMIDAZOLE IN 2 METHYL IMIDAZOLE BY REVERSE PHASE LIQUID CHROMATOGRAPHY
    11 October 2012
    SIMULTANEOUS DETERMINATION OF IMIDAZOLE IN 2 METHYL IMIDAZOLE BY REVERSE PHASE LIQUID CHROMATOGRAPHY

    About Authors:
    Chandorkar J.G.
    Head, Analytical development laboratory
    Indofil Industries Limited
    Thane.
    jchandorkar-icc@modi.com

    Abstract-
    2 methyl Imidazole is the active raw material in the manufacturing of  Micanazole Nitrate, Ketokenazole type Active pharma ceuticals. Imidazole is the Impurity which get form during manufacturing of 2Methyl Imidazole.
    The Proposed Method is to determine the Impurity as well as Content in 2 Methyl Imidazole.

  • EFFECT OF ALCOHOLIC EXTRACT OF ALOE VERA ON DRUG INDUCED MODEL OF COLITIS
    7 October 2012
    EFFECT OF ALCOHOLIC EXTRACT OF ALOE VERA ON DRUG INDUCED MODEL OF COLITIS

    About Authors:
    A.D.Taranalli, Dilip Patoliya*, Sonal Chanchlani, Vishal Patel, Keyur Makadiya
    Department of Pharmacology and Pharmacokinetics,
    K.L.E. University’s College of Pharmacy, Belgaum.
    *Dilip_patoliya@yahoo.com

    ABSTRACT:
    Ulcerative colitis is a chronically recurrent inflammatory bowel disease of unknown origin in which oxidative stress has been implicated. The main aim of the present study was to evaluate possible protective effect of alcoholic extract of Aloe Vera against the drug induced model of colitis in rats. Induction of colitis by colonic administration of drugs(Acetic acid, Indomethacin and TNBS) cause severe microscopic inflammation of colon after 24 hrs of administration of one of any above  inducing agent, as assessed by the macroscopically. Microscopically colonic tissue showed ulceration, oedema and inflammatory cell infiltration. Biochemical estimation studies revealed decrease in serum levels of lactate Dehydrogenase (LDH) may be due to increase in Colonic concentration of tumor necrosis factor α (TNF- α) and neutrophill infiltration index. Oxidative stress was indicated by reduced glutathione concentration (GSH) as well as increase in levels of lipid peroxidase (LPO) and myeloperoxidase (MPO) activity in colonic tissue. Pretreatment with Aloe Vera extract at a dose of (400mg/kg/day, orally),10 days before induction of colitis increase level of serum LDH and GHS, while colonic concentration of LPO and MPO was increased. It also showed decrease in macroscopic score. The findings of the present study provide evidence that Aloe Vera extract may be beneficial in inflammatory bowel disease.

    [adsense:336x280:8701650588]

  • EFFECT OF ETHANOLIC EXTRACT OF ISAPGOL AGAINST DIET INDUCED UROLITHIASIS IN RATS.
    1 October 2012
    EFFECT OF ETHANOLIC EXTRACT OF ISAPGOL AGAINST DIET INDUCED UROLITHIASIS IN RATS.

    About Authors:
    *SOUMYA G., SRINIVAS S., NAVEEN KUMAR M., KRISHNA MOHAN C.
    Department of Pharmacology,
    St. John College of Pharmacy, Warangal,
    Andhra Pradesh, India-5067371.
    *sre_varma123@yahoo.co.in

    Introducion:
    Kidney stone formation or urolithiasis is a complex process that is a consequence of an imbalance between promoters and inhibitors in the kidneys. Even though the technological developments in the present medical practice the formation and growth of renal calculi continues to afflict humankind. Though various kinds of stone have been identified, calcium stones are the most common in human as well as rats. The rat experimental models of Calcium oxalate urolithiasis, induced by ethylene glycol alone, or in combination with other drugs such as ammonium chloride are often used to study the pathogenesis of kidney crystal deposition. The urolithiasis was induced by administration of gentamycin and calculi producing diet (5% ammonium oxalate in standard rat pellet feed) for 28 days to rats’ results in hyperoxaluria, Calcium oxalate crystalluria, and occasional deposition of CaOx crystals in the kidney.

  • FORMULATION AND EVALUATION OF OCULAR INSERT OF ACYCLOVIR
    24 September 2012
    FORMULATION AND EVALUATION OF OCULAR INSERT OF ACYCLOVIR

    About Authors:
    Mistry G. S*, Patel S. D, Tank H. M
    Matushree V. B. Manvar College of Pharmacy
    Dumiyani, Rajkot.
    *Gaurav_mistry123@yahoo.com

    ABSTRACT
    Acyclovir is an Anti-viral drug, widely used in the treatment of Ocular herpes simplex. Ophthalmic insert of acyclovir formulated using Methyl cellulose (MC A4CP), polyvinylpyrrolidone (PVP K30) and polyvinyl alcohol as polymers and glycerin use as plasticizer by solvent casting method with aim of increasing the contact time, achieving sustained release drug. The prepared ophthalmic insert were evaluated for uniformity of thickness, weight uniformity, drug content, % moisture absorption, % moisture loss, folding endurance and surface pH. In vitro drug release of formulated batches was performed using Modified Franz Diffusion cell. A 32 full factorial design was applied to systematically optimize the ocular insert. FTIR spectroscopy was performed to study the drug interaction effect in formulation using KBr disc method. On the basis of all physicochemical parameters and in vitro drug release studies, and overall Desirability, the formulation (F8) was found to vary significantly depending on the type of polymers used and their combinations and it was selected for sterility, stability, ocular irritancy study. The result of invitro diffusion study of formulation exhibited non-fickian in nature. From stability studies inserts were remained stable both physically and chemically. The formulation was found to be practically nonirritant in ocular irritation studies using hen's egg chorioallantoic membrane.

  • EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC ROOT EXTRACT OF ALLOPHYLUS SERRATUS.
    19 September 2012
    EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC ROOT EXTRACT OF ALLOPHYLUS SERRATUS.

    About Authors:
    A.SRILAKSHMI*, AMRUTHA R.E, N.KRISHNA SREE
    Department of Pharmacology
    P. Rami Reddy Memorial College of Pharmacy,
    Andhra Pradesh, India.
    *amrutharamapuram@gmail.com

    Abstract:
    Present study was aimed to evaluate the anthelmentic activity of ethanolic extract of Allophylus serratus against Indian earthworms Pheritima posthuma. The various concentrations of ehanolic extract of Allophylus serratus were tested for paralysis time and death time of the worms. Results revealed that all the concentrations of Allophylus serratus possess anthelmentic activity in a dose dependant manner. Potency of the test samples was found to be inversely proportional to the time taken for paralysis/death of the worms. The activities were comparable with the reference drug Albendazole(20mg/ml). Among the various concentrations of extracts 400mg/ml dose was found to possess promising anthelmentic activity in comparison to other extracts like 100mg/ml,200mg/ml. Phyto constituents of ethanolic extract of Allophylus serratus were found to be Flavonoids, Phenolic compounds,steroids,tri-terpenoids, Glycosides. The present study therefore justifies its use in the folklore remedies as an anthelmentic drug of natural origin.

  • FORMULATION DEVELOPMENT AND EVALUATION OF LANSOPRAZOLE DR ODT
    18 September 2012
    FORMULATION DEVELOPMENT AND EVALUATION OF LANSOPRAZOLE DR ODT

    About Authors:
    Mr. Rajat Kumar Pandeya
    M. Pharm., Dept. of Pharmaceutics,
    Noida Institute of Engg. & Technology
    Greater Noida, India.
    rajatkumar.pandeya@gmail.com

    ABSTRACT :
    Oral disintegrating tablets (ODTs) have received ever-increasing demand during the last decade, and the field has become a rapidly growing area in the pharmaceutical industry. Upon introduction into the mouth, these tablets dissolve or disintegrate in the mouth in the absence of additional water for easy administration of active pharmaceutical ingredients. The popularity and usefulness of the formulation resulted in development of several ODT technologies. This review describes various formulations and technologies developed to achieve fast dissolution/dispersion of tablets in the oral cavity. In particular, this review describes in detail FDT technologies based on lyophilization, molding, sublimation, and compaction, as well as approaches to enhancing the ODT properties, such as spraydrying, moisture treatment, sintering, and use of sugar-based disintegrants. In addition, taste-masking technologies, experimental measurements of disintegration times, and clinical studies are also discussed.

  • SURVEY ON DIABETES AND STANDARDIZATION OF POLYHERBAL FORMULATION
    16 September 2012
    SURVEY ON DIABETES AND STANDARDIZATION OF POLYHERBAL FORMULATION

    About Authors:
    1Shambhawi, *Sai Saraswathi V
    1*Pharmaceutical Chemistry Division,
    School of Advanced Sciences,
    VIT-University, Vellore-632014,
    Tamilnadu, India.
    1shambhawi06@yahoo.co.in, * v.saisaraswathi@vit.ac.in

    ABSTRACT :
    Standardization of polyherbal formulation is important to validate the quality of drugs and to ensure that the consumers are getting medication which guarantees purity, safety, potency and efficacy. The present paper reports standardization of traditional ayurvedic liquid polyherbal antidiabetic formulation (Sucrogen) and diabetes survey for retrieving the information on medication along with the lifestyle of diabetic population. Sucrogen was standardized based on ayurvedic pharmacopeia physico-chemical properties, preliminary phytochemical tests, organoleptic characters, stability studies, microbial studies, TLC, HPLC, heavy metal estimation by AAS and flame photometry to fix the quality standard of this drug. Invitro anti-diabetics activity of the drug was determined using alpha amylase Inhibitory method.These studies resulted in a set of diagnostic characters essential for its standardization. The phytochemical constituents found to be present in raw materials used for the preparation of Sucrogen possibly helps in achieving the desirable therapeutic efficacy of the ayurvedic formulation.
    OBJECTIVE
    To do a general survey on diabetes and to standardize the polyherbal formulation consumed by the population of Jharkhand for the determination of purity and quality of drug

  • METHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF DEFLAZACORT IN TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY
    14 September 2012
    METHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF DEFLAZACORT IN TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY

    About Authors:
    Kapil Sharma*1, Subhash Gupta2, Priyanka Sharma1
    1Yaresun Pharmaceutical Pvt. Ltd.,India.
    2Oasis test house ltd.
    jaipur-302006,rajasthan,india.
    *pharma_kapil@rediffmail.com

    ABSTRACT
    This paper describe a simple, precise and economical spectrophotometric method for the quantitative determination of Deflazacort(DFCT) in tablet dosage form. Method is based on the estimation of DFCT in aqueous acetonitrileat 246 nm. Beer’s law was obeyed in the concentration range of 4-14 µg/ml. The accuracy of the method was assessed by recovery studies and was found to be 99.38±0.15 for DFCT. Results of the analysis were validated statistically so that it can be used for routine analysis of DFCT in tablet dosage form.

Subscribe to Research Articles