Pharmaceutical Analysis Articles

22 March 2013

DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR ASSAY OF AZITHROMYCIN IN POWDER FOR ORAL SUSPENSION

About Author:
Swapna.G*
Department of pharmaceutical Pharmaceutical & Quality Assurance,
Nirmala College of Pharmacy, Mangalagiri, Atmakuru, Guntur -522 203.
*swapna.goday.gs@gmail.com

Abstract
A simple, precise and accurate reversed phase liquid chromatographic method has been developed for the assay of azithromycin in powder for oral suspension. The chromatographic separation was achieved on a Asahipak ODP 40 E(250 mm × 4.6 mm, 5 μm) analytical column. A mixture of methanol–ammonium dihydrogen phosphate (0.05M) (30:70, v/v) (pH 9.0) was used as the mobile phase, at a flow rate of 1.5 mLmin^-1 and detector wavelength at 210 nm. The retention time of azithromycin was found to be at 8.0 min. The validation of the proposed method was carried out for specificity, linearity, accuracy, precision, robustness and stability indicating assay. The linear dynamic range is from 382–1208 μgmL^-1 for azithromycin. The percentage recovery obtained for azithromycin is 101.0%. The developed method can be used for pharmaceutical dosage form and in process testing.
19 March 2013

PRELIMINARY CHARACTERIZATION OF ROCK SALT IN PRESENCE OF CITRIC ACID BY FTIR SPECTROSCOPY

ABOUT AUTHOR:
BINDU.S
ANNAMACHRYA COLLEGE OF PHARMACY,
RAJAMPET,KADAPA Dist, A.P, INDIA.
sirasalabindupharmacy@gmail.com

INTRODUCTION
Rock salt, also known as halite is the naturally occurring crystalline salt. It is the mineral form of sodium chloride or the table salt that we use. However, rock salt does not look like the regular table salt. Instead, it naturally forms in large isometric crystals. Moreover, rock salt can be white like table salt or it can be red, orange, yellow or blue in color. The color of rock salt basically depends on the amount, and the kind of impurities present in it. Along with sodium, rock salt contains many other minerals including, calcium, zinc, iron, magnesium, potassium, copper and several other trace minerals. Like table salt, rock salt too has great many uses. rock salt in massive bags for the purpose of keeping down ice on the roads in the winter.
12 March 2013

SPECTROPHOTOMETRIC ESTIMATION OF TOLPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN SYNTHETIC MIXTURE BY DUAL WAVELENGTH METHOD

ABOUT AUTHORS:
Hiral H. Patel*, Paresh U. Patel,
Department of Pharmaceutical Analysis, Center for Health and Science Studies,
Ganpat University, Ganpat Vidyanagar – 384012,
Mehsana, Gujarat, India.
*patel.hiral2210@gmail.com

ABSTRACT
This method describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic methodfor the simultaneous determination of tolperisone hydrochloride (TOL) and diclofenac sodium (DIC) in bulk and synthetic mixture. In this study, dual wavelength spectroscopic method was used for simultaneous determination of tolperisone hydrochloride and diclofenac sodium using the absorbance difference at two wavelengths. The absorbance differences of 257 nm and 306.20 nm were selected for the estimation of TOL and the absorbance differences of 243.40 nm and 265.40 nm were selected for estimation of DIC. The method was found to be linear (r2>0.999) in the range of 2- 18 μg/ml for tolperisone hydrochloride. The linear correlation was obtained (r2>0.997) in the range of 2- 18 μg/ml for diclofenac sodium. The limit of determination was 0.66 and 0.27 μg/ml for tolperisone hydrochloride and diclofenac sodium respectively. The limit of quantification was 2.00 and 0.83 μg/ml. The method was successfully applied for simultaneous determination of tolperisone hydrochloride and diclofenac sodium in binary mixture.
10 March 2013

A REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PHARMACEUTICAL TECHNOLOGY

ABOUT AUTHORS:
Rajani Pathuri^*, M.Muthukumaran, B.Krishnamoorthy, Amreen Nishat
Montessori Siva Sivani Institute of Science & Technology-College of pharmacy
Mylavaram, Vijayawada, Andhrapradesh-521230
*rajani.prakash4@gmail.com

ABSTRACT
Analytical methods development and validation play important roles in the discovery, development and Manufacture of pharmaceuticals. Method development is the process of proving that an analytical method is acceptable for use to measure the concentration of an API in a specific compounded dosage form which allow simplified procedures to be employed to verify that an analysis procedure, accurately and consistently will deliver a reliable measurement of an active ingredient in a compounded preparation. The analytical method validation is essential for analytical method development and tested extensively for specificity, linearity, accuracy, precision, range, detection limit, quantization limit, and robustness. In summary, analytical method development and validation allows to confirm that an accurate and reliable potency measurement of a pharmaceutical preparation can be performed.
5 March 2013

A CRITICAL REVIEW ON CALIBRATION OF ANALYTICAL INSTRUMENTS

About Authors:
Manoranjan Thunuguntla*, B.Krishnamoorthy, M.Muthukumaran, Amreen Nishat, Vasu Naik
Montessori Siva Sivani Institute Of Science & Technology College Of Pharmacy,
Mylavaram, Krishna District,
Andhra Pradesh
* manozph@gmail.com

Abstract¹:
Analytical instruments are used for specific analysis of drugs and pharmaceuticals. So, regular performance verification is made to ensure that instrument used in the analytical purpose should be properly calibrated and validated “to demonstrate that it is suitable for its intended purpose”. This article is prepared in the sense to get all the information on calibration of basic analytical instruments in laboratory and industrial scale of scientific institutions. The scope of this article is to get the procedures of calibration of analytical instruments at here. Calibration of instruments with their procedure and precautions of Thermometer, Ph Meter, Karl Fisher, Polarimeter, Conductivity Meter, Tablet Fraibilator, Hardness Tester, Disintegration Test Apparatus, Dissolution Test Apparatus, Potentiometer and U. V. Spectrophotometer are mentioned below.
1 March 2013

SIMULTANEOUS ESTIMATION OF GLIMEPIRIDE, PIOGLITAZONE HYDROCHLORIDE AND METFORMIN HYDROCHLORIDE BY DERIVATIVE SPECTROPHOTOMETRY METHOD

About Authors:
Wagh Vinod. T.
Dept. of Pharmaceutics,
DCS’s. A.R.A. College of Pharmacy, Nagaon,
Dhule-424005 MH. India
wagh.vinod517@gmail.com
25 February 2013

EVALUATION OF QUALITY OF FRESH SOYA-BEAN OIL AVAILABLE IN BANGLADESH

ABOUT AUTHORS:
MR. Zubair Khalid labu^*1, Md. Abdul Bake², Razia Sultana^3

1,3Department of Pharmacy, World University of Bangladesh, Dhaka 1205, Bangladesh,²Department of Pharmacy, Gono University, Savar, Dhaka 1344, Bangladesh.
* zubairlabu@yahoo.com

ABSTRACT
Hydrogenated soya-bean oil is the product obtained by refining, bleaching, hydrogenation and deodorisation of oil obtained from seeds ofGlycine soja Sieb. and Zucc. andGlycine max (L.) Merr. (G. hispida (Moench) Maxim.). Soya-bean oilis a vegetable oil extracted from the seeds of the soybean. It is plant based and less harmful saturated fats and offers nutrition and health related benefits. The main objective of this work has been done for the evaluation and analyzing the quality of soya-bean oil available in local market. For the examination of soyabean oil six fresh samples of different sources were collected randomly from the market. British pharmacopoeia (BP) specifications were used for the determination of acid, peroxide and iodine value. The results were compared with the standard specified by Bangladesh standard and testing institute (BSTI). After three months study we observed that physical examination of all the samples were complies with the specification and chemical examination such as, In case of peroxide value, all the samples were found to comply with the specification and only two (S-5 and S-6) of them have comparatively higher value but remain within the limit. But In case of iodine value, five samples were within the limit, and only one sample (S-6) was out of specification. After over third month analysis we observed that all the sample value were higher than from first month study. The consumers which have to take this spurious product must be careful to take this because it may be harmful if adulterated.
27 January 2013

DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD OF DRONEDARONE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL FORMULATION

About Authors:
Gedam V. K., Shrivastav S. R., Raut N.A.
Department of Pharmaceutical Sciences RTM, Nagpur University, Amravati Road,
Nagpur- 440033.
*vkgedam8@hotmail.com

ABSTRACT
The present research work discusses the development of a UV spectrophotometric method for Dronedarone hydrochloride. Simple, accurate, cost efficient and reproducible spectrophotometric method has been developed for the estimation of Dronedarone hydrochloride in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement at maximum wavelength (λmax) at 290nm using methanol as a solvent. The percentage recovery of Dronedarone hydrochloride ranged from 98.48 to 98.96% in pharmaceutical dosage form. Beers law was obeyed in the concentration range of 2-30μg/ml having line equation y = 0.0397x - 0.0060 with correlation coefficient of 0.9998. Results of the analysis were validated statistically and by recovery study.
17 January 2013

BIOPHARMACEUTICAL ANALYSIS NEED AND PROCESS INVOLVED IN PRELIMINARY TREATMENT OF BIOLOGICAL SAMPLES FOR USING LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROSCOPY(LC-MS/MS): A REVIEW

About Authors:
*S.B.Muthu Vadivel, R.Suresh Kumar¹, A.Tamil Selvan², R.Suthakaran³
Department of Pharmaceutical Analysis and Quality Assurance
Teegala Ram Reddy College of Pharmacy
13 January 2013

A REVIEW ON INSTRUMENTATION OF THERMAL ANALYSIS METHOD: DTA, DSC

About Authors:
Bhupender Kumar*, Assit. Prof. Prasant Beniwal, Monish Sharma, Ramchandra
Seth G.L. Bihani S.D. College of Technical Education
(Institute Of Pharmaceutical Sciences And Drug Research),
Sri Ganganagar, Rajasthan
*bhupendra.nimiwal@gmail.com

Abstract:
Thermoanalytical methods essentially techniques that are based entirely on the concept of heating a sample followed by well-defined modified procedures, such as : gravimetric analysis, differential thermal analysis (DTA) and differential scanning calorimetry (DSC). Thermogravimetric analysis measured weight change, differential scanning calorimetry measured heats and temperature of transitions and reactions, differential thermal analysis (DTA) measured temperatures of transitions and reactions.

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