Biochemistry Articles

11 July 2018

INVITRO STUDIES ON THE EFFECT OF TERMINALIA ARJUNA IN ADIPOCYTE 3T3 - L1 CELL LINES

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ABOUT AUTHORS
Ranjitha B¹, Dinesh Bhaskar¹, Balasubramanian Sathyamurthy²
¹Department of Biochemistry,
Reva Institute of Science and Management, Bangalore, Karnataka, India
² Department of Biochemistry,
Ramaiah College of Arts, Science and Commerce, Bangalore, Karnataka, India

ABSTRACT:
The leaves of Terminalia Arjuna are commonly known as Arjuna. It is used as traditional medicinal plant distributed throughout the greater part of the Indian Peninsula along rivers and found in Sub-Himalayan tract, Chota Nagpur, Orissa, West Bengal, Punjab, Deccan and Konkan it has many medicinal properties like anti bacteria, anti inflammatory and used for the treatment of Asthama, Bronchities. These leaf extracts are found to have antioxidant, anticancer and antidiabetic activities. Out of many constituents arjunolic acid is the active principle which is responsible for antidiabetic effects. The present work was aimed to study the effect of Terminalia arjuna. Methanolic extracts on adipocyte 3T3-L1cell line and found the phytochemical compounds of Terminalia arjunaleaves have more inhibitory scavenging and more antidiabetic activities

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11 September 2013

Require Assistant Professor/Associate Professor in Smriti College of Pharmaceutical Education

SCOPE was established in the year 1999, under the banner of B. R. Nahata Smriti Sansthan in the memory of great social leader, renowned parliamentarian and educationalist, Late "Shri Bhanwarlalji Nahata". The institute is solely dedicated to Noble Profession of Pharmacy.
22 May 2013

PREPARATION OF ANTI HUMAN IgG – HRP CONJUGATE BY WESTERN BLOT TECHNIQUE

About Authors
^*1M Prasad Naidu, ²T Madhu Chaithanya, ³N Mallikarjun Rao, ⁴Muneer Bhanu , ⁵Dr Madhu Sudan Reddy
¹Medical Biochemistry, NMCH
²Medical Pharmacology, NMCH
6 March 2013

STAGE IV CANCER: BATTLE NOT LOST YET !

About Author:
Dr. Vishwas B. Chavan
Ph.D. (Biochemistry)
Andheri (East), Mumbai – 400069
vishwaschavan2003@yahoo.co.in

Abstract:
The word “Cancer” is still synonymous with death for common people and the “stage IV cancer”, which is typically known as “last stage cancer”, is loss of hope even for medical community.
Many times patient presents to clinic, when cancer had already crossed II^{nd or} III^rdstage. This is mostly due to the reason that cancer may not have any symptoms at initial stages. Even if there are any symptoms, they can be mild and vague. Further rising cost of healthcare forced many patients, particularly women, to delay approach to physician. Therefore it is not uncommon for cancer patients to hear sentences like, “You are too late”, “You should have come earlier” etc. which is more depressing. This is a humble attempt to give the these patients some ray of hope.
So, in this 21^st Century, is it necessary, that cancer should be equal to death all the times, even when it has reached stage IV? To get answer of this question, we need to first know what stage IV cancer is and what its effects on human body are.
As most people know, cancer or malignancy means uncontrolled growth of any cell of the body. The mechanism which controls the growth of cells is lost in malignant cells. Cancer can occur in any cell of body and hence any organ can be affected by cancer, though cancers of some organs are more prevalent in some populations.
30 December 2012

CLINICAL SPECTRUM OF CITRULLINE-A NON ESSENTIAL AMINO ACID PRESENT IN WATERMELON: AN OVERVIEW

About Authors:
Gaurav Singh^*1, Prof. Satyanand Tyagi², Patel Chirag J³, Patel Pinkesh³, Tarun Parashar¹, Soniya¹
¹*Himalayan Institute of Pharmacy and Research, Rajawala, Dehradun, Uttarakhand, India-248007.
²President & Founder, Tyagi Pharmacy Association (TPA) & Scientific Writer (Pharmacy), Chattarpur, New Delhi, India-110074.
³Department of Pharmaceutics, Maharishi Arvind Institute of Pharmacy, Mansarovar, Jaipur, Rajasthan, India-302020.
*gaurav.hipr@gmail.com, +91-8057832184/9236167104

ABSTRACT:
Citrulline is a non-essential amino acid, meaning that the body can manufacture it from other nutrients. Within the body, citrulline is converted to the amino acid L-arginine i.e. citrulline acts as a precursor of amino acid arginine. Some of the proposed uses of citrulline supplements are based on raising levels of arginine. Citrulline also plays a role in a physiological process called “the urea cycle,” in which toxic ammonia is converted to urea. Citrulline boosts our energy levels by lowering blood lactate concentration. It also enhances the process of elimination of ammonia. Although citrulline is not coded for by DNA directly, several proteins are known to contain citrulline as a result of a posttranslational modification.

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26 November 2012

MASTER PIECE FOR METABOLIC PATHWAYS : THE ENZYME

About Authors:
*C.P. Meher, S.P.Sethy, B.Pochaiah
Department of pharmaceutical Bio-Chemistry,
Maheshwara college of Pharmacy, chitkul (V),
Isnapur “X” Road, patancheru, Hyderabad.
*chaitanyameher84@gmail.com

ABSTRACT :-
“Enzyme are the catalyst of the living world ! protein in nature, and in action in specific ,rapid and accurate; huge in size but with small active centers; highly exploited for disease diagnosis in lab centers”[1] Enzyme promotes & control the conversion of the complex carbohydrates, fats & protein of our body into simple substances which the intestine can absorb & also the various reaction by which these simple substances are used in the body for building up new tissue or producing energy. The enzyme are not broken down or changed in the process . they are as potent at the end of the reaction at the beginning & very small amounts can effect the conversion of large quantity of material . they are the true catalyst.[2] They are actively take part in every metabolic pathways that occur in our body. This review is concern with comparative study of the various enzyme related with various metabolic pathways as well as the drugs associated for inhibition to give respective pharmacological responses.
12 September 2012

ANALYSIS OF SODIUM LEVELS IN JUNK FOOD BY FLAME PHOTOMETER

About Authors:
P.Abisha^1*, D.ANANTHA LAKSHMI²
¹ II M.Sc Biochemistry,
² HEAD OF THE DEPARTMENT
DEPARTMENT OF BIOCHEMISTRY
ST.PIOUS X DEGREE AND PG COLLEGE FOR WOMEN, NACHARAM, HYDERABAD
*godsbloved@yahoo.in

ABSTRACT
Sodium is an essential element for all animal life (including human) and for some plant species. In animals, sodium ions are necessary for regulation of blood and body fluids, transmission of nerve impulses, heart activity, and certain metabolic functions. Sodium is the chief cation in extracellular compartment of animal body.
Sodium plays a vital role not only in human body but also in various food types. Sodium chloride is the principal source of sodium in the diet, and is used as seasoning and preservative, such as for pickling and jerky; most of it comes from processed foods.
The sodium content in various foods can be analyzed by using Flame Photometry. Flame photometry also called flame atomic emission spectrometry is a branch of atomic spectroscopy in which the species examined in the spectrometer are in the form of atoms.
The samples used for analysis are junk foods which have become a common trend among all generations. A total of 15 junk foods were processed and analyzed for estimating the sodium content in them by Flame Photometry.
Based on the analysis the samples were categorized into low, moderate and high sodium containing foods. The analysis helps in creating awareness among all the generations to reduce the sodium intake in order to prevent problems like hypertension, hyponatremia, hypernatremia and associated medical complications in their future.
12 September 2012

CHARACTERISATION OF MARKER CHROMOSOMES

About Authors:
Masani Jigneshkumar Naranbhai
School Of Science
Biomedical Technology
Jiwaji University, Gwalior
jigneshmasani11@gmail.com

ABSTRACT
A supernumerary marker chromosome is an extra structurally abnormal chromosome of unknown origin. Various factors contribute to the difficulty of clarifying the phenotypicrisks of super numerary marker chromosomes (SMC), including differences inthe size, structure, and origin of marker chromosomes.

All marker chromosomes do not contribute the same risk of recurrence. Therefore characterization of the marker chromosomes is of utmost importance for Genetic Counseling. Different molecular techniques can be used to identify the chromosome material present in the markers. In the present retrospective data analysis of 15,000 karyotypes of all patients referred to the Center of Medical Genetics, Sir Ganga Ram Hospital from 1998 to 2012. For constitution chromosome abnormality detection, we found fifteen (0.01%) patients had presence of the SMC. Of these fifteen, eight markers had been characterized after FISH was introduced in the year 2004. This contributed to personalized Genetic Counseling and help for future pregnancies.
6 September 2012

A REVIEW ARICLE ON SYNTHESIS AND BIOLOGICAL PROPERTY OF SOME NOVEL BUTENOLIDE DERIVATIVES

About Authors:
Rajender Kumar^1*,Dr.Sukhbir L.Khokara²,
¹Department of Pharmacy, Manav Bharti University, Solan (H.P.) 173229
²Institute of pharmaceutical sciences, Kurukshetra University,
Kurukshetra, Harayana-136119
*rajkaushal13@gmail.com

ABSTRACT
Butenolides (furanones) consist of unsaturated γ- lactone which occur in plants as glycosides. There is a group of steroids which contain α,β unsaturated butenolide ring in the side chain such steroids are known as cardenolides. The presence of γ - lactone ring contain natural products like Goniobutenolides A & B ,Digitoxigenin and lignans. These compound have biological properties like antibiotic, antitumor, anticonvulsant, antimicrobial and medicinal uses like fungicides, bactericides and allergic inhibitor.
27 May 2012

BIOCATALYSIS IN ORGANIC SOLVENT SYSTEM

About Authors:
Chhavi Bartaria^*, Javed Ali Khan
Institute of Biomedical Education and Research,
Mangalayatan University,
Aligarh.
*cbartaria@gmail.com

Abstract
Biocatalysis involving certain enzymes like NHase, Nitrilase, Tannase and Amidase produces certain commercially important compounds .NHase are used for the synthesis of certain compounds such as acrylamide and nicotinamide. Thermostable Nitrilase from the Rhodococcus strains is used for the modification of acrylic fiber surface. The activity of amidase enzymes is five times increased and inhibited with the use of Co²⁺and Hg^2+, 2-mercaptoethanol, dithiothreitol, and ethylene diaminetetraceticacid respectively. Furthermore the production of amidesis done as a by the help of a nitrilase-catalysed reaction of a heterolytic amino nitriles by a enzyme. For the hydrolysis of Gallic acid esters and hydrolysable tannis the tannase is use as catalyst and this gallic acid is produced by the hydrolysis of methyl gallate by fungal tannase, the tannase activity is also shown by lactic acid bacteria and the nitrilase is used for the modification of acrylic fibers by the help of a reaction media with some parameters like time, enzyme activity and its formulation.