Pharmaceutical Analysis

ABOUT AUTHORS:
Arshad Hala
Seth G. L. Bihani S. D. College of Technical Education,
Institute of Pharmaceutical Sciences & Drug Research,
Gaganpath, Sri Ganganagar, Rajasthan -335001, India

ABSTRACT:
HPTLC is one type of planar chromatography and most advanced form of instrumental TLC. Now a day, HPTLC is more useful than TLC and HPLC. Because HPTLC is independent of sample application, chromatogram development, detection, etc. it is not only instrumental TLC but entire concept that include widely standardize methodology based on validated method. It is instrument controlled by software. In this review article, we discussed about which type of instrument used in HPTLC, complete HPTLC methodology, How HPTLC better than TLC.

Gandhi Institute of  Technology and Management,  popularly known as GITAM was founded in 1980 by an inspired group of eminent intellectuals and industrialists of Andhra Pradesh led by Dr. M.V.V.S.Murthi, Former Member  of  Parliament  and popular philanthropist.   Their mission was to set up a  world-class technology &nbsp

About Authors:
Patel Amit, Hala Arshad, Beniwal Prashant
Seth G. L. Bihani S.D. College of Technical Education,
Institute of Pharmaceutical Sciences and Drug Research,
Sri Ganganagar, Rajasthan, INDIA

ABSTRACT:
The HPLC detectors are used to detect the solute present in the eluent comes from the HPLC column. There are various types of detectors available, but Detector selection in HPLC system is most valuable part in the solute detection. After comparison of all detector, find out which is the best detector for our solute analysis? Then Detector selection was carried out.  Comparisons of all detectors are done on the basis of the sensitivity of the detector for that particular solute.

About Authors:
Tarun Patel , Prof. Dr. Vipin Kukkar, Nilesh Sovasia
Seth G.L. Bihani S.D. College of Technical Education,
Institute of Pharmaceutical Sciences and Drug Research,
Sri Ganganagar, Rajasthan, INDIA

INTRODUCTION
When we speak of a molecule as being raised to a higher electronic level we mean that an electron has been changed from one orbital to another orbital of higher energy. This electron can be of any kinds we have encountered –a σelectron, a π electron or an n electron. In Ultraviolet region we are confined only to the excitation of the comparatively loosely held n and πelectrons.²

Jaipur National University (JNU), Jaipur came into existence in the year 2007. Two professional institutes of the seedling Group, established in 2002, with several technical & professional programmes of studies, were merged with the University to form a major part of the University.

About Authors:
Bhawana Kapoor^*1, Vishnukant Rai², Sonu Sharma^3
1Seth G.L. Bihani S.D. College of Technical Education, Sriganganagar, Rajasthan, India
²Shri Ramnath Singh College of Pharmacy, Gormi, Bhind, M.P., India
³NIMS Institute of Pharmacy, Jaipur, Rajasthan, India

ABSTRACT
Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.

About Authors:
Kalpesh N. Patel¹*, Jayvadan K. Patel^2
1 Research Scholar, Jodhpur National University, Jodhpur,
Rajshathan, India
² Department of Pharmaceutical Technology,
Nootan Pharmacy College,
Visnagar (GUJARAT), India.

Abstract
Two simple spectrophotometric methods have been developed for simultaneous estimation of pyrimethamine and sulphadoxine from tablet dosage form. Method-I simultaneous equation method involves the measurement of absorbances at two wavelengths 215 nm (λmax of pyrimethamine) and 254 nm (λmax of sulphadoxine) in methanol and Method-II first order derivative spectroscopic method involves the measurement of absorbances at two wavelengths 250 nm (λmax of pyrimethamine) and 220.5 nm (λmax of sulphadoxine); The linearity lies between 5-30 µg/ml for both pyrimethamine and sulphadoxine for all the two methods. The accuracy and precision of the methods were determined and validated stastically. All the methods showed good reproducibility and recovery with % RSD less than 2. All method were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of pyrimethamine and sulphadoxine in bulk and combined dosage form.

The college "Saraswati School of Pharmacy" is located at- Ranela, Mahesana. This college runs B. Pharm course in the college campus. The college is run by Shree B. M. Patel Education Trust. This is one of the largest educational campus running professional institutions in the University, to which it is affiliated with. It offers educational course.