About Authors:
Bhawana Kapoor*1, Vishnukant Rai2, Sonu Sharma3
1Seth G.L. Bihani S.D. College of Technical Education, Sriganganagar, Rajasthan, India
2Shri Ramnath Singh College of Pharmacy, Gormi, Bhind, M.P., India
3NIMS Institute of Pharmacy, Jaipur, Rajasthan, India
ABSTRACT
Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.