RESISTANCE OF SERRATIA MARCESCENS (SPKD15) TO VARIOUS ENVIRONMENTAL STRESS CONDITIONS: EFFECT ON CELL VIABILITY AND PRODIOGISIN PRODUCTION
{ DOWNLOAD AS PDF }
ABOUT AUTHORS
1PRATYUSH KUMAR DAS*, 2SMRUTIPRAGNYA SAMAL, 3RATIKANTA SAHOO, 4PRASANT KUMAR SABAT
1,2,3Centre for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India – 751003
4School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India – 751003
*pratyushdas@soa.ac.in
ABSTRACT
Serratia marcescens, a gram negative bacillus is generally related to hospital acquired infections. Production of red pigment (Prodiogisin) by the bacterium is an important characteristic feature and has been reported to exhibit certain antimicrobial property. The resistance of the bacteria towards several classes of antibiotics makes it one of the most notorious pathogen. The work aimed to evaluate the resistance of Serratia marcescens (SPKD15) under various environmental stress conditions (temperature, pH, Salt concentration and UV). Effect of these stress conditions on the cell viability and production of prodiogisin was analysed. The strain was able to sustain up to temperature of 40°C, Salt (NaCl) concentration of 7%, pH up to 10 and could withstand UV radiations up to 2 minutes. However, the prodiogisin production was negatively affected and finally inhibited at all the conditions of environmental stress(Temperature = 35°C, pH = 4 and 9, Nacl concentration = 4% and UV exposure of 15 seconds). This indicates that prodiogisin may act as a protective mechanism for the bacterium under stress. Further, intracellular antimicrobial was obtained from the UV exposed culture and compared with the antimicrobial obtained from the normal culture. The antimicrobial obtained from UV exposed culture showed decreased antimicrobial effect with inhibition diameter ranging between 2 mm to 13 mm (± S.D) as compared to the antimicrobial obtained from the normal culture. An inhibition diameter ranging between 4 mm to 22 mm (± S.D) was obtained in case of the normal culture which may be attributed to the loss of pigmentation. The study highlights the resistance of the bacterium to various environmental stresses. Keeping the degree of pathogenicity of the bacterium in mind, eradication of the same is quite difficult and must be looked upon seriously.
The “Central Government Health Scheme” (CGHS) for the Central Govt. employees and their 
Department of Science & Technology (DST) was established in May 1971, with the objective of promoting new areas of Science & Technology and to play the role of a nodal department for organising, coordinating and promoting S&T activities in the country. The Department has major responsibilities for specific projects and programmes like Formulation of policies relating to Science and Technology, Matters relating to the Scientific Advisory Committee of the Cabinet (SACC) and etc.
Indian Institute of Chemical Technology, Hyderabad is a premier R&D Institute 
Panacea Biotec is a leading research based Health Management Company. We are engaged in research, development, manufacture & marketing of Vaccines, Bio-Pharmaceuticals and Pharmaceuticals (Rx & OTC) of international quality and Health Services. All Panacea Biotec plants are accredited by international agencies and they follow current Good Manufacturing Practices, as per WHO guidelines.Panacea Drugs Pvt. Ltd. was set up in the year 1984, with a commitment to make novel and innovative medicines affordable to the masses a dream of healthy India, and got listed in 1995 as Panacea Biotec Ltd. 
National Institute of Biologicals an autonomous Institution under the Ministry of Health & Family Welfare (MOHFW)-Government of India, was set up in January, 1992 to act as the national control laboratory for assuring the availability of high standard and good quality biological products for consumption in India and for exports. It was set up as a society (vide No. S-22590) under the Societies Registration Act 1860. laid down in Bye-Laws of the institute, for quality control of indigenously produced and imported biological products. Initial financial assistance in setting up the institute was provided by USAID and OECF - Japan.
The Public Health Foundation of India (PHFI) is a not-for-profit public private initiative that has collaboratively evolved through consultations with multiple constituencies including Indian and international academia, state and central governments, multi & bi-lateral agencies and civil society groups. PHFI is a response to redress the limited institutional capacity in India for strengthening training, research and policy development in the area of Public Health. The PHFI focuses on broad dimensions of public health that encompass promotive, preventive and therapeutic services, many of which are frequently lost sight of in policy planning as well as in popular understanding.
This is a question we’ve asked ourselves over and over. It’s how we at Medtronic have made hearts beat stronger and longer. It’s how we’ve pushed the limits of what technology can do in and for the body. It’s how we will continue to alleviate pain, restore health and extend life. Rising costs, an aging population, and the burden of chronic disease impact us all. And no single entity in the healthcare system can solve these challenges alone. That’s why we’re stepping forward. That’s why we’re prepared to put the full power of our technologies, services, resources — and our people — to work with new partners, in new ways, to usher in a new era in healthcare.
Abbott India Limited is a subsidiary of Abbott Laboratories, USA, a global, diversified health care company devoted to the discovery, development, manufacture and marketing of pharmaceutical, diagnostic, nutritional and hospital products. The company now employs approximately 70,000 people and markets its products in 130 countries worldwide.
Across the globe, Lilly employees work to discover and bring life-changing medicines to those who need them, improve the understanding and management of disease, and give back to communities through philanthropy and volunteerism. We were founded in 1876 by Colonel Eli Lilly, a man committed to creating high-quality medicines that meet real needs. More than a century later, we are passionate about building on this precedent in our continued pursuit to make life better for individuals, communities, and the world around us.
Pondicherry University is outstanding among the Central Universities in India. Teaching and research are its primary functions as in other Central Universities. In addition, it has also affiliated colleges located in Puducherry, Karaikal, Mahe, Yanam and the Union Territory of Andaman & Nicobar Islands. The Union Territory of Lakshadweep is also expected to have a college affiliated to Pondicherry University in the near future.
Amrita Institute of Medical Science (Amrita Hospital) brings together a dedicated team of physicians, nurses, and other healthcare professionals to provide the highest standards of medical treatment. Our full range of primary and specialty care medical services enables cross-specialty consultation, which assures outstanding treatment for each patient.
Indian Institute of Science Education and Research Pune is a premier autonomous Institution established by the Ministry of Human Resource Development, Government of India, for promotion of high quality science education and research in the country. 
Global Hospitals Group is one of the fast growing chains of multi super specialty tertiary & quaternary care Hospitals in India offering healthcare services of international standards. Conferred with many prestigious Health Care excellence awards and recognized as the most promising Healthcare chain in India, Global Hospitals Group, with over 2000 beds, has world class hospitals strategically located in the metro cities of Chennai, Bangalore, Hyderabad & Mumbai.
