JANUARY 2016 ARTICLE LIST >>
PharmaTutor (January- 2016)
Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 4, Issue 1)
Received On: 02/09/2015; Accepted On: 07/09/2015; Published On: 01/01/2016
AUTHOR:
Israt Jahan Bulbul*, Khadiza-tul-Kubra, Mohita Chowdhury Shimu
Department of Pharmacy,
Southeast University Banani, Dhaka, Bangladesh
* israt_jahanb872@yahoo.com
ABSTRACT: The main aim of this study was to find out the antibacterial, cytotoxic and antioxidant activities of n-hexane, chloroform and ethyl acetate extracts of T. cucumerina (Cucurbitaceae). Disc diffusion technique was used for in vitro antibacterial screening against gram positive, gram negative human pathogenic bacteria. Here kanamycin disc (30 μg/disc) was used as standard. The chloroform and the n-hexane extract of T. cucumerina showed moderate antibacterial activity with the average zone of inhibition 7-13 mm and 7-9 mm respectively. The brine shrimp lethality bioassay method was used to determine the cytotoxic activity and vincristine sulphate was used as positive control. Among the extractives the chloroform soluble fraction demonstrated the highest cytotoxic activity with LC50 17.09 μg/ml which indicates the compounds present in the chloroform extract are promisingly cytotoxic. Antioxidant activity test of the crude extracts were assessed by means of DPPH free radical scavenging method where ascorbic acid was used as standard. The ethyl acetate fraction of T. cucumerina showed strongest antioxidant activity with IC50 value of 52.18 μg/ml. Incase of phenolic content, the n-hexane, chloroform and ethyl acetate extracts of T. cucumerina revealed 18.79, 31.33 and 29.04 mg of GAE / gm of extractives, respectively.
How to cite this article: Bulbul IJ, Kubra KT, Shimu MC; Antibacterial, Cytotoxic and Antioxidant Activities of n-Hexane, Chloroform and Ethyl Acetate extracts of Trichosanthes cucumerina leaves; PharmaTutor; 2016; 4(1); 42-46
[ABSTRACT WITH CITATION] [VIEW AS HTML]
REFERENCES:
1.Saboo SS, Thorat P, Tapadiya GG, Khadabadi SS; Distribution and Ancient-recent Medicinal Uses of Trichosanthes species; International Journal of Phytopharmacy; 2012; 2(4); 91-97
2. Adebooye OC; Phytoconstituents and anti-oxidant activity of the pulp of snake tomato (Tricosanthes cucumerina); African Journal of Traditional; Complimentary and Alternative Medicines; 2008; 5(2); 173-179.
3. Ojiako OA, Igwe CU, The Nutritive; Anti-Nutritive and Hepatotoxic Properties of Trichosanthes anguina (Snake Tomato) Fruits from Nigeria; Pakistan Journal of Nutrition; 2008; 7(1); 85-89.
4. Jiratchariyakul W, Frahm AW; Cucurbitacin B and dihydrocucurbilacin B from Trichosanthes cucumerina; J. Pharm. Sci.; 1992; 19(5); 12.
5. Swamy MJ, Komath SS, Nadimpalli SK; Identification of histidine residues in the sugar binding site of snake Figure 1: Determination of LC50 values for standard and crude n-hexane, chloroform and ethyl acetate extracts of leaves T. cucumerina from linear correlation between logarithms of concentration versus percentage of mortality. Figure 2: Determination of IC50 value for standard and crude n-hexane, chloroform and ethyl acetate extracts of leaves of T. cucumerina from linear correlation between concentrations (μg/ml) versus percentage of scavenging of DPPH. 45
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gourd (Trichosanthes anguina) seed lectin; Biochem Mol Biol Int; 1998; 44(1); 107-116.
6. Yadava RN, Syeda Y; An isoflavone glycoside from the seeds of Trichosanthes anguina; Phytochemistry; 1994; 36(6); 1519-1521.
7. Yusuf M, Chowdhury JU, Wahab MA, BegumJ; 1994; Medicinal Plants of Bangladesh; BCSIR; Dhaka.
8. Hariti M, Rathee PS; Antibacterial activity of the unsaponifiable fraction of the fixed oil of Trichosanthes seeds; Asian journal of chemistry; 1995; 7(4); 909-911.
9. Kolte RM, Bisan VV, Jangde CR, Bhalerao AA; Anti-inflammatory activity of root tubers of Trichosanthes cucumerina (LINN) in mouse's hind paw oedema induced by carrageenan; Indian Journal of Indigeneous Medicines; 1997; 18(2); 117-21.
10. Kongtun S, Jiratchariyakul W, Mongkarndi P, Theppeang K, Sethajintanin I, Jaridasem S, Frahm AW; Thai, Cytotoxic properties of root extract and fruit juice of tricosanthes cucumerina; J Phytopharm; 1999; 6(2); 1-9.
11. Kar A, Choudhury BK, Bandyopadhyay NG; Comparative evaluation of hypoglycaemic activity of some Indian plants in alloxan diabetis rats; Journal of Ethnopharmacology; 2003; 84(1); 105-108.
12. Arawwawala M , Thabrew I, Arambewela L; Antidiabetic activity of Trichosanthes cucumerina in normal and streptozotocin–induced diabetic rats; Int. J. of Bio. and Che. Sci.; 2009; 3(2); 287-296
13. Kirana H, Srinivasan B; Tricosanthes cucumerina improves glucose tolerance and tissue glycogen in non insulin dependent diabetis mellitus induced rats; Indian Journal of Pharmacology; 2008; 40(3); 103-106.
14. Rahuman AA, Venkatesan P; Larvicidal efficacy of five cucurbitaceous plant leaf extracts against mosquito species; J. of Parasitol Res; 2008; 103(1); 133-139.
15. Kumar SS, Kumar BR. & Mohan GK; Hepatoprotective effect of Trichosanthes cucumerina Var cucumerina L. on carbon tetrachloride induced liver damage in rats; Journal of Ethnopharmacology; 2009; 123(2); 347-350
16. VanWagenen BC, R Larsen, JH Cardellina, D Randazzo, ZC Lidert, C Swithenbank; Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri; J Org Chem; 1993; 58(2); 335-337.
17. Meyer B.N., N.R. Ferrigni, J.E. Putnam, L.B. Jacobsen, D.E. Nichols and J.L. Mclaughlin; Brine shrimp: a convenient general bioassay for the active plant constituents; Planta Medicine; 1982; 45(5); 31-34.
18. Zhao, G.X., Y.-H. Hui, J.K. Rupprecht, J.L. McLaughlin, and K.V. Wood, Additional bioactive compounds and trilobacin, a novel highly cytotoxic acetogenin, from the bark of Asimina triloba; Journal of Natural Products; 1992; 55(3); 347-356.
19. Sadhu SK.; E Okuyama, H Fujimoto and M Ishibashi; Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities; Chemical & Pharmaceutical Bulletin 2003; 51(5); 595-598.
20. Brand-Williams W, Cuvelier ME, Berset C; Use of free radical method to evaluate antioxidant activity; Lebensm Wiss Technology; 1995; 28(1); 25-30
21. Skerget M, Kotnik P., Hadolin M., Hras AR, Simonic M, Knez, Z; Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem; 2005; 89(2); 191-198.
22. Majhenic L, kerget MS, et Knez Z; Antioxidant and antimicrobial activity of guarana seed extracts; Food Chem; 2007; 104(3); 1258-1268.
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