Spectroflourimetric Method for the Estimation of Darifenacin in Pure and Pharmaceutical Formulations
About Author: P. Sai Praveen*, G. Devala Rao, B. Anupama, V. Jagathi
K. V. S. R Siddhartha College of Pharmaceutical Sciences,
Vijayawada - 520 010.
Abstract:
A simple and sensitive spectroflourimetric method has been developed for the estimation of Darifenacin in pure and pharmaceutical dosage forms. Beers law was obeyed in the range of 1 – 5 µg/ml in methanol at an excitation wavelength (λex) of 292nm and an emission wavelength (λem) of 314nm with good correlation coefficient of 0.9995. The limit of detection (LOD) and limit of Quantification (LOQ) for this method are 0.138 and 0.418 µg/ml respectively and the relative standard deviation of intra-day precision was 0.71% and inter-day precision was 1.07%. Stability studies with respect to time and temperature were also carried out. The results obtained were in good agreement with the labelled amounts of the marketed formulations. This method has been statistically evaluated and found to be accurate and precise.
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