Biotechnology Articles

16 March 2016

EMERGENCE OF NOVEL PEPTIDE MOLECULAR CLASS AS ANTIBIOTICS

{ DOWNLOAD AS PDF }

ABOUT AUTHORS
Akansha Bhandari¹, Nitisha Bhandari^2*

1Department of Lifesciences,
Shri Guru Ram Rai Institute of Technology and Science, Patel Nagar, Dehradun, India
² Department of Biotechnology, Graphic Era University, Dehradun, India
*nitishabhandari89@gmail.com

ABSTRACT
Modern era diseases bring more challenges and hurdles to the rational drug targeting. However chemotherapy is the most affected area as most reports are related to antibiotics resistance. Although some successes are accounted as new molecules and new structures are keep on synthesizing or either isolated. One such example is Teixobactin which is isolated for Eleftheria terrae, which was found to be active against Staphylococcus and Mycobacterium bacteria. The story of Teixobactin isolation could open new horizon and opportunistic ways for future designing of useful drugs against these resistance bacteria. In the similar context we tried to compile the relevant paper that was published in nature in 2015 into a commentary based critical review.
3 February 2016

EXTRACTION AND PURIFICATION OF NUCLEIC ACID USING CBNP & PCIA TECHNIQUE

{ DOWNLOAD AS PDF }

About Author
Nirav Soni
Department of Quality Assurance,
A-One Pharmacy College,
Anasan, Ahmedabad, Gujarat, India
nirav_sonic@yahoo.com

ABSTRACT
Isolation of Nucleic acid easily by Solid Phase Extraction (SPE) and this approach, using commercially available Extraction of nucleic acid column-based kits, requires no toxic chemicals and is a rapid and consistent method for concomitant protein extraction. It is a modern technique useful for separation of Nucleic acid which is most reliable, less time consuming and separation of impurities and continuity of reactive products using listed techniques like column-based nucleic acid purification, Nucleic acid methods& ethanol precipitation, DNA separation by silica adsorption. Buffer choice is significant to completely solubilized all proteins in the sample. This technique provides a simple and effective way to analyze protein and nucleic acids simultaneously from the same sample not affecting yield and quality.
11 January 2016

BIOTECHNOLOGICAL/BIOLOGICAL PRODUCTS: ABOUT ITS STABILITY TESTING

{ DOWNLOAD AS PDF }

ABOUT AUTHORS
Vivek P. Chavda^1*, Dharmesh Shaliya², Bhargav Patel³, Ashish Gabani⁴
¹Department of Pharmaceutics, B.K. Mody Government Pharmacy College, Rajkot, Gujarat, India
²Quality Assurance, Intas Pharmaceuticals Ltd., Ahmedabad, Gujarat, India
³Quality Assurance, Zydus cadila pharmaceuticals Ltd., Ahmedabad, Gujarat, India
⁴ Intas Biopharmaceuticals Ltd., Ahmedabad, Gujarat, India
* vivek7chavda@gmail.com

ABSTRACT
The drug delivery systems have made some of the technological advances especially in the case of biopharmaceuticals. When one talks about biotechnological product stability of drug product becomes main culprit due to dynamic nature of drug molecule. This review is grafted using ICH and other regulatory guidelines to provide an overview to stability testing of such molecules.
30 March 2015

BREAKTHROUGHS IN EPIGENETICS

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Shashi Shekhar Anand, Navgeet, Balraj Singh Gill*
Centre for Biosciences,
School of Basic and Applied Sciences,
Central University of Punjab, Bathinda, India
gillsinghbalraj@gmail.com

ABSTRACT
The word ‘epigenetic’ was first coined by Conrad Waddington in 1946. It deals with functionally relevant modifications to the genome that do not include a change in the nucleotide sequence. Till date observation has focused on the functions of genome sequences and how their regulation occurs. The emerging epigenetic changes and the interactions between cis-acting elements with protein factors plays a central role in gene regulation as well as give insight to various diseases. To evaluate the crosstalk of DNA and protein by taking account of the whole genome, one new evolving technique which is called as ChIP-chip, works on the principle of combining chromatin immunoprecipitation with microarray. ChIP-chip has been recently used in basic biological studies and may be improved further and can be useful for other to aspects, like human diseases. Now a days large amount of discoveries by ChIP-chip and other high-throughput techniques like this may be connected with evolving bioinformatics to add to our knowledge of life and diseases.
5 September 2014

A REVIEW ON SINGLE USE DISPOSABLE TECHNOLOGY FOR RECOMBINANT PROTEIN MANUFACTURING

ABOUT AUTHOR
Madhusudan P Dabhole
Group Manager – BioProcess,
Richcore Life Sciences Ltd, Bangalore, Karnataka, India
madhav888@rediffmail.com

ABSTRACT
The manufacturing of recombinant products by fermentation and purification in stainless steel vessels has seen the transition from small scale to large scale and further to single use disposable technology. The requirement to develop and modulate the process has arisen from the cost and manufacturers need to move the facility on mobile platforms. The review describes the strategies and considerations for Single Use Disposable Technology. Recombinant proteins are widely used for treatment of various diseases and disorders. Single Use Disposable Technology makes it promising to produce and formulate these proteins from bench scale to commercial level in a shorter span of time so that it can reach the physician and patients.
29 May 2014

DNA MICROSATELLITES: A REVIEW

{ DOWNLOAD AS PDF }
2 February 2014

BIOTECHNOLOGY FOR A BETTER WORLD

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Nirav.R. Soni
M.Pharm, A-One Pharmacy College,
Anasan, Ahmedabad-382330, India
nirav_sonic@yahoo.com

ABSTRACT:
Pharmaceutical Biotechnology is not a source of energy, but a scientific method that provides tools to produce energy based entirely on modern biotechnological techniques, as to date encompass a wider range of altogether newer medicinal compounds, e.g., antibiotics, vaccines development and monoclonal antibodies (MABs) that may now be produced commercially using well-defined, optimized and improved fermentative methodologies. In fact, genetic engineering has brought in a sea change by virtue of the directed construction of microorganisms resulting in a plethora of newer life-saving drugs. Focus on a variety of Research areas including health/medicine, food science, environmental science and agri- science and also bioenergy development.
7 November 2013

DRUG DESIGNING : A REVIEW

ABOUT AUTHOR:
Muhammed Mujahed
Master’s of Science in Biotechnology.
SRTM University.
mujubiotech2011@rediffmail.com

INTRODUCTION:
Drug design is an integrated developing discipline which portends an era of ‘tailored drug’. It involves the study of effects of biologically active compounds on the basis of molecular interactions in terms of molecular structure or its physico-chemical properties involved. It studies the processes by which the drug produce their effects, how they react with the protoplasm to elicit a particular pharmacological effect or response how they are modified or detoxified, metabolized or eliminated by the organism.

Disposition of drugs in individual region of biosystems is one of the main factors determining the place , mode and intensity of their action . The biological activity may be “positive” as in drug design or “negative” as in toxicology. Thus drug design involves either total innovation of lead or an optimization of already available lead. These concepts are the building stones up on which the edifice of drug design is built up.

The drug is most commonly an organicsmall molecule that activates or inhibits the function of a biomolecule such as a protein, which in turn results in a therapeutic benefit to the patient. In the most basic sense, drug design involves the design of small molecules that are complementary in shape and charge to the biomolecular target with which they interact and therefore will bind to it. Drug design frequently but not necessarily relies on computer modeling techniques. This type of modeling is often referred to as computer-aided drug design. Finally, drug design that relies on the knowledge of the three-dimensional structure of the biomolecular target is known as structure-based drug design.
12 August 2013

MONOCLONAL ANTI BODIES FOR CANCER TREATMENT

ABOUT AUTHOR:
Vivek P. Chavda
Department of Pharmaceutics, B.K. Mody Government Pharmacy College,
Rajkot – 360003, Gujarat (India)
vivek7chavda@gmail.com
9 July 2013

SINGLE CELL PROTEIN AND BAKER’S YEAST

ABOUT AUTHOR:
Rajesh G. Dobariya
shree M.&N. Virani Science College,
Rajkot
drajesh47@gmail.com

ABSTRACT
Single cell protein typically refers to source of mixed protein extracted from pure or mixed culture of algae, yeast, fungi or bacteria. The microbes which are used for single cell protein production must be non-pathogenic to plants, animals and man. Good nutritional value, easily and cheaply produced on scale, toxin free, fast growing, easily to separate from the medium and to dry. They have many silent feature. Biomass production is ordinarily carried out in continuous mode to maximize yields and economic scale. The raw material of this process is very cheap because we used molasses, whey, gas, oil etc. For a substrate. So SCP is waste to best. The molasses and various salts including ammonium and phosphate salt contain of the baker’s yeast. The yeast are used for the production of SCP. The baker’s yeast is useful to as and they create disadvantages also the SCP and baker’s yeast very useful for organism.

(adsbygoogle = window.adsbygoogle || []).push({});