APR 2015 ARTICLE LIST >>
PharmaTutor (April- 2015)
Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 3, Issue 4)
Received On: 22/01/2015; Accepted On: 17/02/2015; Published On: 01/04/2015
AUTHORS: Fatema Nasrin*, Saikat Ranjan Paul, Sonia Zaman, Sabiha Ferdowsy Koly
Department of Pharmacy,
Southeast University, Banani, Dhaka, Bangladesh
*nasrin_0209@yahoo.com
ABSTRACT
In the present study the antimicrobial & cytotoxic activity of crude methanolic extracts of leaves and stems of VignaMungoLinn. Hepper (Family-Leguminosae)were studied. Antimicrobial activity was tested against eleven important pathogenic bacteria including both gram positive and gram negative bacteria and two common fungi. The bacteria are B. megaterium, B. subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Salmonella paratyphi, S. typhi, Shigella boydii, S. dysenteriae Vibrio mimicus and V. parahemolyticus. Disc diffusion technique was used for invitro antibacterial and antifungal screening. Here kanamycin disc (30mg /disc) was used as standard for antibacterial study. The extracts showed antimicrobial activity against most of the bacterial strains with an average zone of inhibition of 10-20mm. The tested fungi are Candida albicans and Aspergillus niger. The extracts showed very good antifungal activity with an average 15 -19 mm zone of inhibition. The methanolic extracts of leaves of V. mungo showed maximum activity (19 mm, zone of inhibition) against Bacillus megaterium (19mm) with Minimum inhibitory concentration (MIC) values of 64mg/ml. The maximum zone of inhibition for the methanolic extracts of stems was found 20mm against Shigellaboydii with MIC values of 64mg/ml. Cytotoxicity test was also studied by Brine Shrimp Lethality Bioassay and compare with LC50 values of standard vincristin sulphate as a positive control. The results illustrated significant cytotoxicity against A. salina, with LC50 0.67μg/ml, 4.52 μg/ml and 3.25 μg/ml for vincristine sulphate as standard, leaves and stems extracts, respectively. Further pharmacological investigations are required to understand the underlying mechanism of these pharmacological activities.
How to cite this article: F Nasrin, SR Paul, S Zaman, SF Koly; Study of Antimicrobial and Cytotoxic activities of Vigna Mungo Linn. Hepper; PharmaTutor; 2015; 3(4); 40-46
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