March 2012

South Eastern Coalfields Limited invites applications from the willing candidates who fulfill required qualification for the following vacant posts. The details and other terms & condition are given below.After the appointment the candidates will have the benefit of other allowances, beside the Basic Salary, VDA, SDA as per rules of Coal India Limited under National Coal Wages Agreement-VIII.

Ultra Beauty Care Pvt. Ltd., is a leading cosmetics manufacturing company based in Aurangabad (Maharashtra) with a state of the art manufacturing plant designed as per the Schedule M of GMP.
The company currently manufactures cosmetics on Loan License basis for leading brands. UBCPL is also involved in development of products to be offered to our exports clients on a regular basis in Europe & Africa.

MOLECULES INVOLVED IN REGULATION OF STEM CELL DIFFERENTITIATION
Cell adeshive molecule E_cadhedrin which replaces oct 4signalling molecule there by converting normal somatic cell into pluripotent

About Authors:
RAVISANKAR.M*¹, SUBASINI.U², ANAND THANGADHURAI.S³, KARTHIKEYAN.S⁴, CHANDRA SEKAR.E.^5
1.Swamy Vivekanandha College of pharmacy, dept of pharmaceutical analysis. Thiruchengode
^2.Swamy Vivekanandha College of pharmacy, dept of pharmacognosy. Thiruchengode
^3.Swamy Vivekanandha College of pharmacy, dept of pharmaceutical analysis. Thiruchengode
^4.Kausikh therapeutics and private limited.chennai
^5.Swamy Vivekanandha College of pharmacy, dept of pharmaceutical analysis. Thiruchengode

ABSTRACT
An isocratic reverse phase high performance liquid chromatographic method for estimation of montelukast sodium and levocetirizine in bulk dosage and in marketed formulations has been devised and validated. The chromatographic separation achieved on shodex c18-4E column (5µm, 250 mm x 4.6 mm) and  acetonitrile: methanol: ammonium acetate buffer (PH- 5.5) in the ratio of 25:55:20 v/v. The flow rate was 1.0 ml/min and the UV detection was identified at 225nm.The retention times for montelukast sodium and levocetirizine was found to be 5.15 min and 3.12 min respectively. The linearity of montelukast sodium and levocetirizine is 10 -50µg/ml with the correlation co efficient 0.99 respectively. The validation parameters such as accuracy, precision, LOD, LOQ, Robustness, ruggedness were performed as per ICH guidelines. This method can be used for routine analysis of montelukast sodium and levocetirizine in bulk and marketed dosage forms.