Pharmaceutical Analysis Articles

23 September 2012

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF IRBESARTAN BY DERIVATIVE SPECTROSCOPY (FIRST ORDER)

About Authors:
Gunjan Kalyani^*1, Vishal S. Deshmukh¹, Pranita Kashyap¹, Yogesh Vaishnav¹, Ram D. Bawankar
¹Shri Rawatpura Sarkar Institute of Pharmacy,
Kumhari, Durg, Chhattisgarh
*kalyani.gunjan@yahoo.in

Abstract
Irbesartan is chemically 2-butyl-3-({4-[2-(2H-1,2,3,4-tetrazol-5-yl) phenyl] phenyl} methyl) -1,3-diazaspiro [4.4] non-1-en-4-one. Irbesartan is an Angiotensin II receptor antagonist effective in the treatment of Hypertension. It is also effective in the treatment of High blood pressure. It is also effective when used alone or in combination with other drugs. Objective of the present study is to develop a simple, sensitive, accurate, precise and rapid first order derivative spectrophotometric method for the estimation of irbesartan in pure form. For the estimation of irbesartan, solvent system employed was 50% v/v aqueous ethanol and wavelength of detection (λ_det) was 237 nm. The linearity was obtained in the range 8 – 18 µg/ml, with a regression coefficient, R²= 1. The LOD & LOQ were found to be 0.5 µg/ml and 1.63 µg/ml respectively. Obtained results showed that there is minimum intra day and inter day variation. The developed method was validated and recovery studies were also carried out. Sample recovery using the above method was in good agreement with their respective labeled claims, thus suggesting the validity of the method and non-interference of formulation excipients in the estimation. First order derivative spectroscopy method is simple, rapid and reproducible and further it can be used for the analysis.
14 September 2012

METHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF DEFLAZACORT IN TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY

About Authors:
Kapil Sharma^*1, Subhash Gupta², Priyanka Sharma¹
¹Yaresun Pharmaceutical Pvt. Ltd.,India.
²Oasis test house ltd.
jaipur-302006,rajasthan,india.
*pharma_kapil@rediffmail.com

ABSTRACT
This paper describe a simple, precise and economical spectrophotometric method for the quantitative determination of Deflazacort(DFCT) in tablet dosage form. Method is based on the estimation of DFCT in aqueous acetonitrileat 246 nm. Beer’s law was obeyed in the concentration range of 4-14 µg/ml. The accuracy of the method was assessed by recovery studies and was found to be 99.38±0.15 for DFCT. Results of the analysis were validated statistically so that it can be used for routine analysis of DFCT in tablet dosage form.
4 September 2012

GC-MS ANALYSIS OF ACALYPHA INDICA LINN

About Author:
Manisha Singh
Sagar Institute of Pharmacy & Technology,
Gandhi nagar, Bhopal (M.P.)
manishamasih85@gmail.com

Abstract
Acalypha indica Linn. consists of dried herb of Acalypha indica Linn. belonging to familyEuphorbiaceae. Within the Euphorbiaceae family, Acalypha indica is one of the biggest and most diverse, with nearly 450 species. Two thirds of its species are found in America, 19 of them in Venezuala, they are mainly used as ornamental, but with the rising of traditional medicine, these species are given medical properties painkillers, laxatives, anti-inflammatory and many others.The GC-MS analysis of methanol and acetone (70 : 30) extract prepared from the whole plant of Acalypha indica linn. revealed the presence of different phytochemicals.
31 August 2012

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LAMIVUDINE BY USING RP-HPLC

About Authors:
Y. Kranthi Kumar*, Y.Prathyuha
Department of Pharmaceutical Analysis,
Venkateshwara Institute of Pharmaceutical sciences
Cheralapally, Nalgonda- 508001
*kk16052@gmail.com

Introduction
The present work deals with the studies carried out on the development and validation of RP-HPLC for the Lamivudine. Now a days the solutions have gained a lot of importance due to greater patient acceptability, increased potency, multiple action, fewer side effects and quicker relief.
29 August 2012

DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF TADALAFIL AND DAPOXETINE HCL IN SOLID DOSAGE FORM

About Authors:
CHIRAG S. RAJPARA*¹, JAWED AKHTAR¹, AMIT KHANDHAR²
1. Department of Quality Assurance, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur-302025, Rajasthan, India.
2. Zydus cadila pharma, moraiya,ahmedabad.
*chiragrajpara2601@gmail.com

ABSTRACT
* A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed for simultaneous estimation of Tadalafil and Dapoxetine HCL, Water Symmetry C-18 (150 x 4.6 mm),5 µ and a mobile phase composed of Buffer : Acetonitrile (65:35)

* The retention time of Tadalafil and Dapoxetine HCL were found to be10.08 and 4.45 min respectively. Linearity was established for Tadalafil and Dapoxetine HCL in the range of 8.0-60 μg/ml and 24.0-180.0 μg/ml respectively. The percentage recovery of Tadalafil and Dapoxetine HCL were found to be in the range of 99.7-100.6% and 98.07-99.09% respectively. The drug was subjected to acid and alkali hydrolysis, oxidation, dry heat and photolytic degradation. The degradation studies indicated, Tadalafil showed degradation in acid and alkali while it was found stable in H₂O_2,photolytic and in presence of dry heat and Dapoxetine showed degradation in thermal and peroxide while it was found stable in rest of parameters . The degradation products of Tadalafil and Dapoxetine HCL in acidic, alkaline conditions were well resolved from the pure drug with significant differences in their retention time values. This method can be successfully employed for the quantitative analysis of Tadalafil and Dapoxetine HCL in bulk drugs and formulations.
25 August 2012

SIMULTANEOUS ESTIMATION OF FUROSEMIDE AND SPIRONOLACTONE IN COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

About Author:
Hardik Patel^*, Sagar Solanki
Department of Pharmaceutical Chemistry,
K.B.Raval College of Pharmacy, Shertha,
Gandhinagar-382423, Gujarat, India.
*patel1928@yahoo.in

ABSTRACT
A new, simple, rapid, accurate, precise and sensitive method has been developed for the simultaneous estimation of Furosemide and Spironolactone in their combined tablet dosage form. The method was carried out on a Hiber C₁₈ column (250 mm×4.6mm, i.d.5 μm) with a mobile phase consisting of acetonitrile: water at a flow rate of 1 ml/min and the detection was carried out at 237 nm. The retention time of Furosemide and Spironolactone was 3.81 min and 7.28 min. respectively. Linearity for Furosemide and Spironolactone were found in the range of 2-10 μg/ml and 5-25 μg/ml respectively. The developed method was validated in terms of linearity, accuracy, and precision, limit of detection (LOD) and limit of quantification (LOQ). The proposed method can be used for estimation of both drugs in their combined dosage form.
25 August 2012

DETERMINATION OF SYNTHETIC INTERMEDIATE OF DICLOFENAC SODIUM IN REACTION MIXTURES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

About Authors:
1) Mr Jaesh N.Jadhav
M.Pharm,Sinhagad Institute of pharmacy,Pune.
2) Mr. J.G. Chandorkar*.
Head Analytical development laboratory,
Indofil Industries limited,Thane
*Jayant.chandorkar@rediffmail.com

Abstract
A present work describes a simple & accurate reversed phase HPLC Method for the simultaneous estimation of Ortho Chlorophenol [OCP], 2,6 – Dichlorophenol [DCP], 1-(2,6-Dichlorophenyl) indolin-2-one [VTCL], 1(2,6-Dichlorophenyl) N-Phenyl, N-Chloro Acetyl, 2,6-Dichloro Aniline [VTDL], 1(2,6-Dichlorophenyl) ether [DCPE], 1(2,6-Dichlorophenyl) amine [DCPA] in Diclofenac Sodium bulk manufacturing. This paper describes a new rapid, easy Isocratic reversed phase HPLC method for the separation and estimation of six intermediates and Diclofenac Sodium. The primary purpose of this study is to compile HPLC data on the determination of these seven products, the compilation of such HPLC data being useful as reference guide.
8 August 2012

Simultaneous Spectrophotometric Determination of Cefixime and Moxifloxacin in Bulk Drug and Drug Formulation by Absorption Ratio Method

About Authors:
Shah Chirag K.*, Umalkar Deepak, Dr. Rajesh KS.
Parul Institute of Pharmacy, Gujarat University,
Waghodia-391760, Dist. Vadodara,
Gujarat, India.
*cks2484@gmail.com

Abstract:
The present manuscript described the simple, rapid, accurate, sensitive, precise and economical Q- absorption ratio method for simultaneous determination of Cefixime and Moxifloxacin in combined tablet dosage form. Absorption ratio method uses the ratio of absorbances at selected wavelengths, one which is isoabsorptive point and other being the λ_max of the one of the components. Cefixime and Moxifloxacin shows isoabsorptive point at 275nm in 0.1N HCl. The second wavelength used is 295nm, which is λ_max of Moxifloxacin. The concentrations of the drugs were determined by using ratio of absorances at isoabsorptive point and at λ_max of Moxifloxacin. The method successfully applied to the Pharmaceutical formulation because no interference from the tablet formulation excipients was found. The results of analysis have been validated statistically and by recovery studies.

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8 August 2012

QUANTITATIVE DETERMINATION OF ASENAPINE MALEATE USING REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

About Authors:
T.R.PARTHASARATHI*, M.VANITHA SRI.
BIOANALYTICAL DEPARTMENT,
QUEST LIFE SCIENCES PVT. LTD, SDF III, MEPZ,
TAMBARAM, CHENNAI- 600 045, INDIA.
*parthu_14@yahoo.co.in

ABSTRACT :
A novel isocratic reverse-phase high performance liquid-chromatography method for determination of asenapine maleate was developed and validated after optimization of various chromatographic conditions. Samples were separated on a waters x-terra C₁₈ (100 mm × 4.6 mm, 3.5 μ) analytical column. The mobile phase used was acetonitrile: 0.1M phosphate buffer (p^H3.2) 65:35%v/v operated at 30 °C column oven temperature was pumped at a flow rate of 1.0 mL min⁻¹ and the column eluents were monitored at a wavelength of 272 nm. When sample was injected into the Finnigan surveyor high performance liquid-chromatography system through Finnigan surveyor auto-sampler injector, separation was achieved within 5.0 min. The present method demonstrated was validated with the acceptable values for selectivity, linearity (within the expected concentration range (10–50 μg mL⁻¹; r² > 0.999)), recovery (>95%), precision (%RSD < 2.0), sensitivity (limit of detection: 1.85 µg mL⁻¹ and lower limit of quantification: 2.34 µg mL⁻¹), robustness, and ruggedness.
11 July 2012

Anti-inflammatory activity oflic extract of R Methanooot of Cissampelos pareira on Carragenin induced rat paw edema

About authors:
Gourab Saha^*1, Pankaja Senapati¹, Narahari Sahu², Dr. Sambit Parida³
1. Department of Pharmaceutics, College of Pharmaceutical Sciences, Mohuda, Berhampur – 2, Orissa, India.
2. Department of Pharmacology, College of Pharmaceutical Sciences, Mohuda, Berhampur – 2, Orissa, India.
3. Department of Pharma analysis, College of Pharmaceutical Sciences, Mohuda, Berhampur–2, Orissa, India.
*gourab.pharma2012@gmail.com

Abstract
This study investigated the anti-inflammatory activity of the methanolic extract of Cissampelos pareira (Abuta) in male albino rats after intramuscular administration. This was done using the carragenin-induced paw edema method. Methanolic extract of Cissampelos pareira showed significant anti-inflammatory activity similar to ibuprofen and indomethacin.