Bioferments in Formula of Personal care Products

The virtues of fermentation within the human body are well known. It is proven good techniques for food, as Kimchi, Sauerkraut, Kefir, Idli & Dosa utilizes this technique. it is also significant for beauty products. The latest trend in Cosmetics particularly Skin Care is fermented beauty products. This concept, originated in Korea, basically involves fermentation of natural ingredients like black tea, ginseng, seaweed, bamboo sap extract, red clove flower, hibiscus, snow lotus and various herbs and flowers.

The cosmetics industry is always looking for innovation, and fermentation products, will likely become the world trend of tomorrow. While mankind has revived the ancient yet scientifically advanced concept of fermentation, it, in turn, can reinvigorate the glow and texture of skin, among other benefits.

Ferments such as probiotics, enzymes, bacteria or yeast have been used in cosmetics for years. Indeed, the enzymes help to maintain our skin microbiome, the cutaneous microbial flora which consists of more than 500 different species of microorganisms that are multiplying every day on our skin.

This variety is crucial in the good balance of the skin and allows it to be better protected against external aggressions. Because, the less varied the skin microbial flora is, the more the unwanted pathologies can settle, including eczema or acne. By forming a protective barrier against external aggressions, inflammation, infections and other skin diseases, the skin microbiome can, in the long term, help the skin to remain better and to be better protected. The microbiome is, indeed, an important part of the skin’s barrier function and plays an essential role in the good health of the epidermis.

A balanced microbiome means that the skin looks healthy, radiant and regenerates itself better. When it’s weak and damaged, it can provoke dryness, sensibility, breakouts and other skin conditions.
In short, the ferments in cosmetics help to stimulate the natural skin defences. They can also be used to purify acne prone skin, to help rebuild the skin barrier of the sensitized skin or to protect the skin from pollution thanks to their antioxidant properties.
Their use has been known for many years in cosmetics, but what is more recent is the use of fermented natural ingredients, not just ferments alone.

Fermented ingredients are gaining a massive following for being more potent, powerful, and super boosting in skin care products.
Bio-fermented products for the cosmetic and personal care applications employs the principles of fermentation, which allows for the isolation and concentration of various phytochemicals. Fermentation has been regarded as both a traditional art and modern science.
Bioavailability of actives is increased by utilizing modern benefits of fermentation. Ingredients that provide increased skin and hair affinity are taking centre stage in the cosmetic industry.

Bio-fermented ingredients incorporated into beauty products can provide increased skin and hair affinity. For cosmetics, fermented ingredients are more symbiotic with the skin and support skin functions without disrupting natural processes making the ingredients less likely to cause sensitivity in skin care applications.

By initiating fermentation, various cosmetic benefits, such as improved bioactive delivery, oxygen uptake, moisturization, and reduction in skin irritation, can be increased and made more readily bioavailable for skin and hair care applications.

Benefit of Fermented ingredients : Indeed, fermenting natural ingredients gives them new properties:
– the fermentation process maintains the integrity of the ingredients.
– a fermented ingredient has a more acidic pH, which has a better affinity with the skin (with its naturally acidic pH),
– the fermentation process transforms the ingredients: during the fermentation, the ferments break down their molecular structure. The fermented ingredients have smaller, Simpler molecules, which allows them to penetrate the skin more deeply and quickly,
– the fermentation adds nutrients such as amino acids and peptides to the ingredient and makes the fermented ingredient richer in antioxidants than its unfermented version,
– the fermented ingredients also have anti-inflammatory properties. Thanks to the fermentation process, they become rich in enzymes, which are able to soothe and prevent skin inflammation. Basically, the enzymes eliminate the “bad bacteria” the skin may contain and replace them with “good bacteria”, this in turn helps to preserve the skin balance.

The use of both types of ingredients, ferments and fermented ingredients has a lot of advantages for the skin because they don’t “work” on the same level. The ferments have an action on the microbial flora of the skin (which is on the surface, in the protective layer of the epidermis) and the fermented ingredients penetrate into the deeper layers of the epidermis and promoting its natural renewal.
Our specialty ingredients research offers a wide and diverse product portfolio including extra potent natural product range which includes Ferment Filtrates of the natural veggies with proven benefits. Kumar Organic Products Ltd., continuously endeavors to anticipate the requirements in the markets and we unveil the new portfolio of Bio-ferments. One of the key modern benefits associated with bio-ferments are that we can incorporate these active ingredients into beauty products because of its increased skin and hair affinity.

Bio-ferment filtrates are water soluble, hence are easy to incorporate in wide range of formulations of leave on & rinse of products in both skin care & hair care sector. Bio-ferments have unique healing properties to repair damaged skin.
Following are the properties of bio-ferment filtrates for skin & hair
a. Antioxidant – the bio-ferments are rich in antioxidant, enhances the free radical scavenging activities, thus delaying the aging of the skin.
b. Moisturising – Bio-ferment filtrates have immense moisturising properties thereby  retaining natural moisture of skin & hair. They heal the cracks in the skin leaving the skin soft & smooth.
c. Vitamins – Natural vitamins in the ferment filtrates improves the texture of the skin & hair.
d. Minerals - In the maintenance of the skin, minerals play a major role. They maintain the texture of the skin, the elasticity & the firmness of skin, revitalizes the skin, balancing the cell activity.
e. Phyto-nutrients – reduces the photo damage, inflammation, oxidative stress & breakdown of extra cellular matrix of the skin.
We are currently marketing our following Ferment Filtrates whereas we are working on various others like Onion (Kopalleum) & Rice (Kopryza)

Kopcarotol (INCI: Bacillus/Carrot root Ferment Filtrate) : COSMOS APPROVED
Carrot is a reservoir of vitamins A, B, C, K and phytonutrients like carotenes and pigments. Introduction of the friendly bacterium Bacillus ginsengisoli into the rich matrix of carrot roots, mobilizes the hard core bio-actives into the solution which is stabilized into a ready to use Active Ingredient.
It is a potent antioxidant that attack free radical to improve skin dullness & aging .It gives radiance and glow to skin as it has tyrosinase inhibiting activity. It also revitalizes and tones the skin, aids collagen production, maintains skin elasticity, prevents wrinkles, Repairs skin tissues, protects skin against harsh sun rays. It Makes hair healthier and strong. It is Water Soluble & effective in the formulations over wide pH range of 4 to 8.

Kopcarotol can be used in various Formulation like Hydrating Face mist, Biphasic Makeup remover, Skin Essence serum, Hydrating body lotions, Hair conditioner, Sun screen lotions.

Kopraditol: (INCI: Lactobacillus/Radish Root Ferment Filtrate) : COSMOS APPROVED
A radish root ferment filtrate obtained as a result of the fermentation of white radish roots by the organism Lactobacillus casei. Radish is known for its beautification values for skin and hair. They are rich in vitamins, phytonutrients and has a cleansing, detoxification properties. But the strong pungent smell of radish prevents one from getting its benefits. To get its benefits and make it available to skin and hair, the essentials of the radish is transferred in to liquid medium through fermentation. The thus obtained filtrate can be directly used in various formulations. Radish ferment filtrates provides you enhanced antioxidant activity for a flawless skin and healthy hair.

Due to high stress, medications & pollution skin tends to dry up, get tanned, damaged & release free radicals. A one stop solution to overcome all these issues is Kopraditol. The antioxidant properties of Kopraditol fights free radicals, scavenges it and thus delays the aging process. It heals the damaged cells, clears the toxins released, and nourishes it. The lost moisture content is restored back giving a suppleness to the skin. It has anti tyrosinase activity which blocks the action of tyrosinase enzyme thus reducing the production of melanin. The reduction in the melanin content in the skin gives a clear tone to the skin. On the regular use of the products with the Kopraditol the skin is well hydrated & nourished with the phyto nutrients of the radish, it gets detanned with a lighter skin tone.
It also helps to have healthier hair by clearing the free radicals of the scalp and nourishing the scalp cells with vitamins and minerals. The hair root and follicle gets healthier and moisturised. Healthier scalp results in healthy hair. It can be used in various formulations like Skin whitening cream, Nourishing Shampoo, Moisturizing lotion, Skin lightening toner, Deodorants
Kopsoyatol (INCI: B.subtilis and S.cerevisiae / Soya Bean Ferment Filtrate)
It is a biologically active product obtained from the dual fermentation of soyabean by Saccharomyces cerevisiae and Basillus subtilis having excellent antioxidant and antiaging activities.

Kopsoyatol is a potent antioxidant with its immense skin nourishing properties. It has active form of phytoharmones & isoflavones, for a youthful nourished skin. . Its flavone content nourishes and moisturises the skin, replenishes the core skin structure with a flawless texture.

The nutritional benefits helps in skin and scalp rejuvenation and moisturises it from within.
Kopsoyatol a water soluble ingredient can be readily used in both emulsion based and water based formulation. A dream ingredient of formulator’s choice is quiet stable in various formulation like Anti-aging serums, Youth retention mousse, Age defying Scalp masks, Refreshing bubble bath, Rejuvenating hand cream.
We, at Kumar Organic Products Research Centre have developed many formulations using these Bio-ferments and these have been tested to prove its goodness on skin & hair.
Following are few formulations, which are tested for its efficacy and are elaborated for its attribute.

Skin Glowing and Radiance cream
This formula is with Carrot Ferment Filtrate (Kopcarotol), having Antioxidant activity and Moisturization.

FORMULATION :

Phase	No	Ingredient	Wt.%	Trade name /supplier	Uses
A	1	Aqua	Q.S	In-house	Solvent
	2	Acrylates/C10-30 Alkyl Acrylate Cross polymer	0.6	Carbopol ultrez 20 /Lubrizol	Rheology modifier
	3	Glycerin	2	S .D .Fine	Humectant
	4	Sodium benzoate	0.5	Ganesh chemicals	Preservative
	5	Potassium Sorbate	0.5	Ganesh chemicals	Preservative
		Sodium gluconate	0.1	S .D .Fine	Chelating agent
B	6	Cetyl alcohol	2	S .D .Fine	Emulsifier
	7	Capric caprylic triglycerides	3	Farmoganic	Emollient
	8	Glyceryl Stearate (and) PEG-100 Stearate	3	Arlacel 165/Croda	Emulsifier
	9	Niacinamide, Acetyl glucosamine , CentellaAsiaticaextract, Morus albabark extract , Pyrus Malusfruitextract , Camellia Sinensis leaf extract, BroussonetiaKazinoki root extract , Tricholoma Matsutake extract , Galactoarabinan, Camellia Japonica leaf	2	Biospectrum	Active
C	10	Lactobacillus / Carrot root ferment filtrate	5	Kopcarotol /KOPL	Skin glowing Moisturizing& Antioxidant
D	11	Snowdrop	0.5	Sonarome	Perfume
E	12	pH adjuster  (NaOH 18 % Sol )	~0.5*	S .D .Fine	pH modifier

PROCEDURE :
1. Weigh phases A & B ingredients separately and heat to 70-80°C.
2. Add phase B  to A with continuous the stirring at 70-80°C.
3. Homogenize the mixture for 5-7mins at 5500rpm.
4. Add phase C to the emulsion at 45˚C.
5. Add phase D with continuous stirring at Room temperature (RT).
6. Add phase E to adjust the pH. Mix well, allow the batch to cool & settle.

PRODUCT SPECIFICATION :

Parameter	Specification
Appearance	White cream/Light amber color
Odor	Characteristic
pH	5.60
Viscosity*	5900cps

PRODUCT WRITE-UP :
This cream with Kopcarotol gives light coverage and soft silky after feel, the skin gets an immediate smoothness and radiance glow.

Formulation efficacy :
The formulation efficacy was determined for antioxidant activity.

The FRAP assay is directly measures antioxidants (or reductants) in a sample compared to other assays measuring inhibition of free radicals.

Antioxidant activity: The formulation efficacy was determined by FRAP (ferric reducing antioxidant power) method. This method relies on the reduction of ferric ion-TPTZ (2,4,6-tri(2-pyridyl)-1,3,5- triazine) by the antioxidants. Thus formed Fe+2-ligand produce intense blue color which is correlated with antioxidant content.
Procedure : One gram of the cream formulation was extracted with 9 ml of 50% methanol, centrifuged and filtered to obtain clear extract. Antioxidant activity of this extract was determined using standard FRAP assay protocol.

Fig 1: Antioxidant activity comparison study of Kopcarotol cream with other marketed creams containing natural extracts as antioxidants.

Results : Kopcarotol cream containing 5% Carrot root ferment filterate as sole antioxidant active ingredient provides higher antioxidant activity when compared to other marketed products with a combination of multiple ingredients like to Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.

Moisturisation / Hydration study
Objective : To Evaluate the Moisturization/Hydration properties of Kopcarotol cream
An in-vivo study was conducted for 24 hours to evaluate the moisturization benefits. Kopcarotol cream results indicate that this material is capable of significantly increasing moisturization compared to the untreated control, placebo & competitor ingredient.
Materials :
Equipment : Multi skin test center (MC 750 Corneometer)
The moisture module provides information about the skin’s moisture content by measuring the conducting properties of the upper skin layers.
10 volunteers M/F between the ages of 25 and 50 and who were known to be free of any skin pathologies participated in this study. MC 750 Corneometer was used to measure the moisture levels on the subject’s volar forearms. The Corneometer is an instrument that measures the moisture content of the skin. Presence of moisture in the skin improves the conductance therefore results in higher readings than dry skin. Higher the levels of moisture content in the skin, higher will be the Corneometer readings. Baseline moisturization readings were taken on day one of the study.
Procedure : Apply 50mg of each test material on volar forearms in 4×4cm. Readings was taken immediately after the application of test materials – initial, after 2, 8 & 24 hours.
For added perspective, measurements of an untreated test site and a site treated with a base cream without Kopcarotol were recorded.
Results : Kopcarotol cream showed high moisturizing capabilities at 5.0% concentration. Please note that each value is an average of three consecutive readings per test site.

Fig 2: results of skin moisture content study of Kopcarotol cream

Discussion : As evidenced in 8 hours efficacy study of Kopcarotol Cream on skin, moisture content of the skin was increased by 55%, when compared to untreated control 30%, Competitor ingredient 47% & base cream 42%. Results indicate that a product with Kopcarotol provides higher moisture content to the skin when compared to the untreated control, base cream& competitor ingredient. Moisturization was maintained 58% after 24hours whereas competitor ingredient 45% & base cream 42%.

Moisturization study comparison against Market products with Kopcarotol

Fig 3 : Skin Moisturization study of Kopcarotol cream in comparison to other marketed products containing natural actives such as Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.

Discussion : Kopcarotol cream containing 5%Kopcarotol as sole active ingredient provides higher moisturization to the skin when compared to other marketed products with a combination of multiple ingredients like to Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.
Results : The study result confirms that a cosmetic formulation with Kopcarotol enhances the skin moisture content.

Skin Lightening Cream
This formula is with Radish Ferment Filtrate (Kopraditol), having Antioxidant activity, Tyrosinase inhibition activity and Moisturization.

FORMULATION :

Phase	No	Ingredient	Wt.%	Trade name /supplier	Uses
A	1	Aqua	Q.S	In-house	Solvent
	2	Acrylates/C10-30 Alkyl Acrylate Cross polymer	0.6	Carbopol ultrez 20 /Lubrizol	Rheology modifier
	3	Glycerin	2	S .D .Fine	Humectant
	4	Sodium benzoate	0.5	Ganesh chemicals	Preservative
	5	Potassium Sorbate	0.5	Ganesh chemicals	Preservative
		Sodium gluconate	0.1	S .D .Fine	Chelating agent
B	6	Cetyl alcohol	2	S .D .Fine	Emulsifier
	7	Capric caprylic triglycerides	3	Farmoganic	Emollient
	8	Glyceryl Stearate (and) PEG-100 Stearate	3	Arlacel 165/Croda	Emulsifier
	9	N butyl resorcinol	0.1	Kopcinol /KOPL	Active
C	10	Lactobacillus / Radish root ferment filtrate	5	Kopraditol /KOPL	Skin lightening booster Moisturizing& Antioxidant
D	11	Snowdrop	0.5	Sonarome	Perfume
E	12	pH adjuster  (NaOH 18 % Sol )	~0.5*	S .D .Fine	pH modifier

*QS to pH 5.5- 6.5

PROCEDURE :
1. Weigh phases A & B ingredients separately and heat to 70-80°C.
2. Add phase B  to A with continuous the stirring at 70-80°C.
3. Homogenize the mixture for 5-7mins at 5500rpm.
4. Add phase C to the emulsion at 45˚C.
5. Add phase D with continuous stirring at Room temperature (RT).
6. Add phase E to adjust the pH. Mix well, allow the batch to cool & settle

PRODUCT SPECIFICATION :

Parameter	Specification
Appearance	White cream/Light amber color
Odor	Characteristic
pH	5.90
Viscosity*	5600cps

Formulation efficacy :
The formulation efficacy was determined for antioxidant activity.
The FRAP assay is directly measures antioxidants (or reductants) in a sample compared to other assays measuring inhibition of free radicals.
Antioxidant activity: The formulation efficacy was determined by FRAP (ferric reducing antioxidant power) method. This method relies on the reduction of ferric ion-TPTZ (2,4,6-tri(2-pyridyl)-1,3,5-triazine) by the antioxidants. Thus formed Fe+2-ligand produce intense blue color which is correlated with antioxidant content.
Procedure : One gram of the cream formulation was extracted with 9 ml of 50% methanol, centrifuged and filtered to obtain clear extract. Antioxidant activity of this extract was determined using standard FRAP assay protocol.

Fig 1 : antioxidant activity comparison study of Skin lightening cream with other marketed creams containing natural extracts as antioxidants.

Results : Skin lightening cream containing 5% Radish root ferment filtrate as sole antioxidant active ingredient provides higher antioxidant activity when compared to other marketed products with a combination of multiple ingredients like to Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.

Tyrosinase inhibition activity:
Tyrosinase inhibition activity: Dopa oxidase activity of mushroom tyrosinase was spectrophotometrically determined. The reduction in activity in the presence of sample was estimated as % inhibition. 
Where:

Fig 2 : comparison of tyrosinase inhibition by Kopraditol and benchmark formulation

Moisturization/Hydration study : P

Objective : To Evaluate the Moisturization/Hydration properties of Skin lightening cream
An in-vivo study was conducted for 24 hours to evaluate the moisturisation benefits. Skin lightening Cream results indicate that this material is capable of significantly increasing moisturization compared to the untreated control, placebo & competitor ingredient.

Materials :
Equipment: Multi skin test center (MC 750 Corneometer)
The moisture module provides information about the skin’s moisture content by measuring the conducting properties of the upper skin layers.
10 volunteers M/F between the ages of 25 and 50 and who were known to be free of any skin pathologies participated in this study. MC 750 Corneometer was used to measure the moisturelevels on the subject’s volar forearms. The Corneometer is an instrument that measures the moisture content of the skin. Presence of moisture in the skin improves the conductance therefore results in higher readings than dry skin.  Higher the levels of moisture content in the skin, higher will be the Corneometer readings. Baseline moisturization readings were taken on day one of the study.
Procedure :  Apply 50mg of each test material on volar forearms in 4×4cm. Readings was taken immediately after the application of test materials – initial, after 2, 8 & 24 hours.     
For added perspective, measurements of an untreated test site and a site treated with a base cream without Kopraditol were recorded.
Results : Skin lightening cream showed high moisturizing capabilities at 5.0% concentration. Please note that each value is an average of three consecutive readings per test site.

Percent (% ) change	T=0	T=2hrs	T=8hrs	T=24hrs
Untreated control	30	30	30	25
Base cream	38	40	42	42
Experimental  ( 5%Kopraditol )	50	53	54	60
Cream with Competitor ingredient 5%	42	46	45	43

Fig 3 : Results of skin moisture content study of Skin lightening cream

Discussion: As evidenced in 8 hours efficacy study of Skin lightening Cream on skin, moisture content of the skin was increased by 54%, when compared to untreated control 30%, Competitor ingredient 45% & base cream 42%. Results indicate that a product with Kopraditol provides higher moisture content to the skin when compared to the untreated control, base cream& competitor ingredient. Moisturization was maintained 60% after 24hours whereas competitor ingredient 43% & base cream 42%.

Moisturization study comparison against Market products with Kopraditol

Fig 4 : Skin Moisturization study of Skin lightening cream in comparison to other marketed products containing natural actives such as Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.
Discussion : Skin lightening cream containing 5%Kopraditol as sole active ingredient provides higher moisturization to the skin when compared to other marketed products with a combination of multiple ingredients like to Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.
Results : The study result confirms that a cosmetic formulation with Kopraditol enhances the skin moisture content.

Anti-Aging Cream
This formula is with Soyabean Ferment Filtrate (Kopsoyatol), having Antioxidant activity, Moisturization and Anti-ageing activity.

FORMULATION :

Phase	No	Ingredient	Wt.%	Trade name /supplier	Uses
A	1	Aqua	Q.S	In-house	Solvent
	2	Acrylates/C10-30 Alkyl Acrylate Cross polymer	0.6	Carbopol ultrez 20/Lubrizol	Rheology modifier
	3	Glycerin	2	S .D .Fine	Humectant
	4	Sodium benzoate	0.5	Ganesh chemicals	Preservative
	5	Potassium sorbate	0.5	Ganesh chemicals	Preservative
	6	Sodium gluconate	0.1	S .D .Fine	Chelating agent
B	7	Cetyl alcohol	2	S .D .Fine	Emulsifier
	8	Capric caprylic triglycerides	3	Farmoganic	Emollient
	9	Glyceryl Stearate (and) PEG-100 Stearate	3	Arlacel 165/Croda	Emulsifier
	10	N-butyl resorcinol	0.1	Kopcinol /KOPL	Active
C	11	Lactobacillus /Soya root ferment filtrate	5	Kopraditol /KOPL	Skin lightening booster Moisturizing& Antioxidant
D	12	Snowdrop	0.5	Sonarome	Perfume
E	13	pH adjuster (NaOH 18 % Sol )	~0.5	S .D .Fine	pH modifier

pH:5.5-6.5

PROCEDURE :
1. Weigh phases A & B ingredients separately and heat to 70-80°C.
2. Add phase B to A with continuous the stirring at 70-80°C.
3. Homogenize the mixture for 5-7mins at 5500rpm.
4. Add phase C to the emulsion.
5. Add phase D with continuous stirring.
6. Add phase E to adjust the pH. Mix well, allow the batch to cool & settle.

PRODUCT SPECIFICATION :

Parameter	Specification
Appearance	White cream / off white cream
Odor	Characteristic
pH	5.60
Viscosity	4800cps

Formulation efficacy :
The formulation efficacy was determined for antioxidant activity & moisturizing property.

Antioxidant activity: The formulation efficacy was determined by FRAP (ferric reducing antioxidant power) method. This method relies on the reduction of ferric ion-TPTZ (2,4,6-tri(2-pyridyl)-1,3,5-triazine) by the antioxidants. Thus formed Fe+2-ligand produce intense blue color which is correlated with antioxidant content.

Fig 1 : antioxidant activity comparison study of kopsoyatol cream with other marketed creams containing natural extracts as antioxidants

Anti-aging activity : Presence of total flavonoids was checked by Aluminium chloride (AlCl3) colorimetric method to estimate the anti-aging property. AlCl3 forms acid stable complexes with the C-4 keto groups and either the C-3 or C-5 hydroxyl group of flavones and flavonols. The complex absorbs at 415 nm, which is correlated to total flavonoids content.
Moisturization/Hydration Assay:

Objective : To Evaluate the Moisturization/Hydration Assay in Kopsoyatol cream
An in-vivo study was conducted for 8 hours to evaluate the moisturization benefits Kopsoyatol cream Results indicate that this material is capable of significantly increasing moisturization compared to the control

Materials :
Equipment : Multi skin test center (MC 750 / Moisture Probe)
The moisture module provides information about the skin’s moisture content by measuring the conducting properties of the upper skin layers
10 volunteers M/F between the ages of 25 and 50 and who were known to be free of any skin pathologies participated in this study. MC 750 Corneometer was used to measure the moisture levels on the subject’s volar forearms. The Corneometer is an instrument that measures the moisture content of the skin. Presence of moisture in the skin improves the conductance therefore results in higher readings than dry skin.  Higher the levels of moisture content in the skin, higher will be the Corneometer readings. Baseline moisturization readings were taken on day one of the study.
Procedure :  Apply 5mg of each test material on volar forearms. Readings was taken immediately after the application of test materials – initial check, after 2 hrs & 8 hrs.
For added perspective, measurements of an untreated test site and a site treated with a base cream without Kopsoyatol were recorded.
Results: Kopsoyatol cream showed high moisturizing capabilities at a 5.0% concentration. Please note that each value is an average of three consecutive readings per test site.

Percent (% ) change	T=0	T=2 Hours	T=8 Hours
Untreated control	30	30	30
Base cream	38	40	42
Experimental  ( 5%Kopsoyatol)	50	51	55
Competitor ingredient	43	44	47

Fig 2 : Results of skin moisture content study of Kopsoyatol cream

Discussion: As evidenced in 8 hours efficacy study of Kopsoyatol Cream on skin, moisture content of the skin was increased by 55%, when compared to untreated control 30% & base cream 42%. Results indicate that a product with Kopsoyatol provides higher moisture to the skin when compared to both the untreated control & base lotion.

Moisturization assay comparison to Market products with Kopsoyatol

Fig 3 : Skin moisturization study of Kopsoyatol Cream in comparison to other marketed products containing natural actives such as Aloe vera, Vit C, Vit E , Green tea extract, Mulberry extract, Galctomyces filtrate, etc
Results: Kopcarotol cream containing 5%Kopsoyatol as sole active ingredient provides higher moisturization to the skin when compared to other marketed products with a combination of multiple ingredients like to Aloe vera, Vit C, Vit E, Green tea extract, Mulberry extract, Galctomyces filtrate, etc.
Conclusion: The study result confirms that a cosmetic formulation with Kopsoyatol enhances the skin moisture content.
Fermented soy can boost the production of hyaluronic acid, which leads to plumper, softer and firmer skin.
From above examples, it can be very well understood that Bioferments, because of its attribute, can contribute a lot to the efficacy of Cosmetics products.
Fermented ingredients are now popular in the world of Cosmetics and Personal Care, thanks to the development of biotechnologies, opening up new horizons for these new favorite ingredients in the cosmetics industry.

NOW YOU CAN ALSO PUBLISH YOUR ARTICLE ONLINE.

SUBMIT YOUR ARTICLE/PROJECT AT admin@pharmatutor.org

FIND OUT MORE ARTICLES AT OUR DATABASE