Monthly archive | PharmaTutor

15 October 2016

Explain Electrophoresis, its principle and factors governing it

Q.5. (c) Explain Electrophoresis, its principle and factors governing it
Ans.5.(c) Electrophoresis: is a technique used to separate and sometimes purify macromolecules - especially proteins and nucleic acids - that differ in size, charge or conformation. As such, it is one of the most widely-used techniques in biochemistry and molecular biology.

15 October 2016

Discuss the principle of separation.

Q.5. (c) Discuss the principle of separation.
Ans.5.(c) The separation of chemical components is performed using chromatographic technique. Chromatography is an analytical technique used for separating a mixture of chemical substances into its components so that these can be identified or analyzed.

15 October 2016

Discuss the principle of GLC

Q.5. (b) Discuss the principle of GLC and its column configuration, detection system & stationary phases.
Ans.5.(b) Principle of GLC Gas chromatography is probably the most utilized of all the chromatographic techniques.

15 October 2016

Write an exhaustive note on size exclusion chromatographic technique and its pharmaceutical applications

Q.5. (b) Write an exhaustive note on size exclusion chromatographic technique and its pharmaceutical applications

15 October 2016

Classify column chromatography techniques

Q.5. (a) Classify column chromatography techniques

15 October 2016

Discuss various pumping systems, columns & detectors in HPLC

Q.5. (a) Discuss various pumping systems, columns & detectors in HPLC. Ans.5. (a) pumping system in HPLC Analytical Pumps (Solvent Delivery System) The requirements for HPLC pumps are as follows: They must be able to generate high pressures, have a pulse free output, deliver flow rates ranging from 0.1 to 10 ml/min, have flow reproducibility’s of 0.5% relative or better and they must be resistant to corrosion by a variety of solvents. Various types of pumping systems are

15 October 2016

Write an exhaustive note on DSA technique

Q.4.(c) Write an exhaustive note on DSA technique, its principle and pharmaceutical applications.

15 October 2016

Explain Index of hydrogen deficiency and its significance

Q.4. (b) Explain Index of hydrogen deficiency and its significance
Ans.4.(b) Index of Hydrogen Deficiency

15 October 2016

Discuss the theory of Mass spectroscopy

Q.4. (a) Discuss the theory of Mass spectroscopy

Ans.4.(a) Theory: Mass spectrometry is the most accurate method for determining the molecular mass of the compound and its elemental composition. In this technique, molecules are bombarded with a beam of energetic electrons. The molecules are ionized and broken up into many fragments, some of which are positive ions. Each kind of ion has a particular ratio of mass to charge, i.e. m/e ratio (value). For most ions, the charge is one and thus, m/e ratio is simply the molecular mass of the ion.

15 October 2016

Discuss non first order spectra in PMR spectroscopy and its resolution by shift reagents, deuterium labeling and other methods.

Q.3. (c) Discuss non first order spectra in PMR spectroscopy and its resolution by shift reagents, deuterium labeling and other methods.