SEX DETERMINATION USING POLYMERASE CHAIN REACTION
About Authors:
Emanual Michael Patelia*, Rakesh Thakur, Jayesh Patel
Department of Pharmacology,
University of Bedfordshire, Luton, LU1 3JU, England.
*ricky.emanual@gmail.com
Abstract
The PCR is widely used technique in sex determination. PCR is also used for the determination of x linked inherited disorders, with help of biopsied embryos into mothers. DNA polymerase enzyme replicates a piece of DNA. Thus, chain reaction occurs and generating multiple copies of it. Polymerase chain reaction is frequently used technique for sex determination. PCR can also be used for detecting the presence or absence of a particular piece of DNA. In this method, we used PCR method for determination of the sex of three unknown bovine samples.PCR techniques have developed to reduce the problems by increasing amplification quality.