May 2011

To the great Guru Nanak who was an apostle of peace, truth and universal brotherhood, there could not be a better tribute on his 500 birth-day than the decision to build a "State of the Art" Hospital & Research Centre in the Metropolis of Bombay. The ailing society and suffering mankind, which Guru Nanak loved the most, sorely need such facilities.
The driving force behind this noble venture are the disciples of the great Guru spearheaded by the Shree Singh Sabha, Bombay.

Abour Author: RJ Pathyusha B
M.Pharm,
T.K.R.C.O.P, JNTU

Reference ID: PHARMATUTOR-ART-1043

ABSTRACT
The present work describes the local anesthetic activity of milky latex obtained from Calotropis procera and its potentiation with epinephrine and pH. The milky latex at a dilution of 1:10, exhibited significant local anesthetic activity. Both epinephrine (5µg/ml) and pH of 7.2 prolonged the duration of local anesthetic activity. The method used is infiltration anesthesia in Guinea pig. In conclusion, the duration of the effect produced by combination of Calotropis procera latex and epinephrine was longer than that by latex alone.

The University Institute of Pharmaceutical Sciences of the Panjab University is a premier institution of Pharmaceutical Education and Research in the country. The institute has covered a long and glorious journey of 76 years starting from Lahore in 1944 and finally settling to the present campus in Chandigarh at the foothills of Himachal.

About Authors: MALLIKARJUNA RAO N.
1.     Research scholar of Jawaharlal Nehru Technological University, Department of Pharmaceutical Analysis, College of Pharmacy, Kakinada, Andhra Pradesh, India.  

Reference ID: PHARMATUTOR-ART-1042

ABSTRACT
Objective:This present study reports for the first time simultaneous quantitation of Amlodipine besylate and Atorvastatin calciumby HPTLC from a combined dosage form.
Methods: Chromatographic separation of the drugs were performed on aluminum plates precoated with silica gel 60 F₂₅₄ used as stationary phase and the chromatogram was developed using Ethyl acetate: Methanol: Ammonia (7.5 : 2 : 0.5 %v/v/v) as mobile phase. Amlodipine besylate and Atorvastatin calcium showed Rf values 0.50 ±0.02 and 0.26 ±0.02 respectively. Densiometric analysis of both the drugs was carried out in the absorbance mode at 365 nm. The method has been successfully applied to tablets and was validated according to ICH Harmonized Tripartite guidelines.
Results: The linearity regression analysis for calibration showed 0.9983 (r²) and 0.9994 (r²) for amlodipine besylate and atorvastatin calcium with respect to peak area and height in the concentration range of 100-500ng/spot and 200-600ng/spot respectively.  The percentage recovery for amlodipine besylate was found to be 101.82 (at 50%), 99.12 (at 100%) and 101.41 (at 50%), 101.71 (at 100%) for atorvastatin calcium. The limit of detection was 30 ng/spot and    60 ng/spot for amlodipine besylate and atorvastatin calcium respectively. The limit of quantification was found to be 100 ng/spot and 200 ng/spot for amlodipine besylate and atorvastatin calcium respectively.
Conclusion: The developed TLC technique is precise, specific and accurate. It was concluded that the developed method offered several advantages such as rapid, cost effective, simple mobile phase and sample preparation steps and improved sensitivity made it specific, reliable and easily reproducible in any quality control set-up providing all the parameters are followed accurately for its intended use.

About Authors: Arun Kumar Dash*, Suchismita Jena, Sudhir Kumar Sahoo
Department of Pharmaceutical Analysis and Quality Assurance
Royal College of Pharmacy and Health Sciences,
Andhapasara Road Berhampur, Odisha

Reference ID: PHARMATUTOR-ART-1040

ABSTRACT
A simple method for the estimation of Pioglitazone in bulk and pharmaceutical dosage forms has been developed. 0.1N HCl was chosen as the solvent system. The λmax was found to be 270nm and all absorbance values were carried out at 270nm. The responses were linear in the range of 5-100µg/ml. The regression equation of the calibration graph and correlation coefficient were found to be y = 0.026x - 0.020 and 0.999 respectively. The %RSD values for both intraday and interday precision were less than 1%. The recovery of the drug from the sample was ranged between 99.36 and 100.49%. The proposed method was validated for precision, accuracy, intraday, interday assay, robustness and ruggedness. Commercial tablets containing 20mg and 40mg of Pioglitazone were analyzed by the proposed method and the results were well within the claimed limits. Furthermore the effect of pH on Pioglitazone was studied.

About Author: D. Gopi Krishna
M.Phil
Tirumala Engineering College
Hyderabad, Andhra Pradesh

ABSTRACT:
Antibiotics are one of the most important commercially exploited secondary metabolites produced by the bacteria and employed in a wide range. Most of the antibiotic producers used today are the soil microbes. Fungal strains and streptomyces members are extensively used in industrial antibiotic production. Bacteria are easy to isolate, culture, maintain and to improve their strains. Microbes are omni present and exist in a competitive environment. Bacillus species being the predominant soil bacteria because of their resistant endospore formation and production of vital antibiotics like bacitracin etc. are always found inhibiting the growth of the other organisms. In the present project a soil bacterium with the antibiotic activity is screened studied for morphological  characters which can provide valuable information about the strain. The inhibitory activity of the organism is checked against some of the important opportunistic microbial flora and inoculated into an appropriate designed media depending on the bacterial requirements, and incubated for 48 hrs at 370C.  The produced compound is extracted by solvent extraction and assayed for its activity. Enhancement in the antibiotic production is studied under various parameters like temperature, pH, carbon source concentration, and Sodium nitrate concentration, which may help in the industrial production. The extracted substance was found effective against the gram positive endospore forming bacilli and gram positive coci. Though a large list of antibiotics are commercially available, the search for the most potential one is still on, and this work may provide some potential information on the antibiotic production and the control of microbial strains.

Objective: The objective of the present study is production extraction and assay of   antibiotics from bacterial and fungal isolates using soil as source.