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PharmaTutor (May- 2014)
ISSN: 2347 - 7881
Received On: 25/03/2014; Accepted On: 01/04/2014; Published On: 01/05/2014
Author: Krutika J. Bhalodiya*, Nehal Ghelani, Darshan Madiya, Shital Faldu
Department of Quality Assurance
Smt. R. D. Gardi B. Pharmacy College, Rajkot
bhalodiyakruti1@gmail.com
ABSTRACT:
UV Spectrophotometric method has been developed for simultaneous estimation of Amiloride (AML) and Torsemide (TSM) in bulk drug and in their combined dosage form by first order derivative. This method utilizes methanol as a solvent and λmax of Amiloride and Torsemide selected for analysis was found to be 248 nm(at ZCP of TSM) and 308 nm (at ZCP of AML) respectively. Linearity was observed in the concentration range of 4-14μg/ml for AML (r2 = 0.999) and 4-20 μg/ml for TSM (r2 = 0.998). The accuracy and precision were determined and found to comply with ICH guidelines. This method showed good reproducibility and recovery with % RSD in the desired range. The proposed methods can be successfully applied for the routine analysis of both the drugs. This method was simple, rapid, accurate, and sensitive.
How to cite this article: KJ Bhalodiya, N Ghelani, D Madiya, S Faldu; Analytical Method Development and Validation of First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Amiloride and Torsemide in their Combined Pharmaceutical Dosage Form; PharmaTutor; 2014; 2(5); 138-143
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REFERENCES:
1. Indian Phamacopoeia, Volume-I & Volume-II, Government of India, Ministry of Health and Family Welfare, Published by The Indian Pharmacopoeia Commission, Ghaziabad. 2010, pp 795,796
2. Rang HP, Dale MM, Ritter JM and Moore PK, Pharmacology, 5th Edn; Elsevier, New Delhi 2006, pp 251,442.
3. The United States Pharmacopoeia-30, NF-25, Asian edition, Rockville, MD: The US Pharmacopoeial Convention, Inc. 2004, pp 113,1088,1869.
4. British Pharmacopoeia, Volume I-II, London: The Department of Health,: HMSO Publication; 2009, pp 2789
5. Ines TM, Pope SG “Simultaneous determination of Amiloride and Furosemide in pharmaceutical formulations by first digital derivative spectrophotometry.” International Journal of Pharmaceutics. 2002 , 249, pp 117-26
6. Laxmhmi VK “Spectrophotometric Determination of Amiloride in bulk and pharmaceutical formulation.” International journal of pharmacy and pharmaceutical science. 2013, 5, pp 975-1491
7. Marothu VK and Dannana GS “Simple Spectrophotometric Determination of Torsemide in Bulk Drug and in Formulations.” Journal of Chemistry. 2008,5, pp 473-478
8. Gupta JA, Kanojiaa GP, Yadava VS, Wakode SR “Development and validation of a UV spectrophotometric method for the estimation of torsemide in bulk and in tablet dosage form.” Journal of chemistry & Pharmaceutical Research 2010, 2, pp 513-517
9. ICH guideline Q2 (R1). Validation of analytical procedures: text and methodology. Geneva: 1996.
10. Beckett AH. And Stenlake JB. Practical Pharmaceutical Chemistry, part-II; 4th Edn; CBS Publisher and Distributors, New Delhi, 2007, pp 275-336
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