JUL 2014 ARTICLE LIST >>
PharmaTutor (July- 2014)
ISSN: 2347 - 7881
Received On: 13/04/2014; Accepted On: 29/05/2014; Published On: 01/07/2014
AUTHORS: Krutika J. Bhalodiya*, Darshan Modiya, Shital Faldu
Department of Quality Assurance
Smt. R. D. Gardi B. Pharmacy College, Rajkot, India
*bhalodiyakruti1@gmail.com
ABSTRACT:
RP-HPLC method was developed for the simultaneous estimation of Amiloride and Torsemide in pharmaceutical dosage form. The separation was achieved by BDS C18 (150 × 4.6 mm, 5 µm) column with Methanol:Phosphate buffer pH 3.6 (10:90 %v/v). Flow rate was maintained at 1.0 ml/ min and UV detection was carried at 288 nm. Retention time for Amiloride and Torsemide was found to be 1.944 min and 8.903 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Amiloride and Torsemide were in the range of 3-7 µg/ml and 6-14 µg/ml respectively. The percentage recoveries obtained for Amiloride and Torsemide were found to be in range of 99.87- 100.80 and 100.1-101.3 respectively. The developed method was validated as per ICH guidelines. Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of Amiloride and Torsemide in pharmaceutical dosage form.
How to cite this article: KJ Bhalodiya, D Modiya, S Faldu; Analytical Method Development and Validation for Simultaneous Estimation of Amiloride and Torsemide in their Combined Pharmaceutical Dosage form by RP-HPLC Method; PharmaTutor; 2014; 2(7); 135-141
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