Opening for M.Sc/ M.Pharm/ Ph.D as Senior Research Scientist in Piramal Life Sciences Limited

Piramal Life Sciences Limited (PLSL) is an independent research-driven drug company that was recently demerged from Piramal Healthcare Limited (Formerly known as Nicholas Piramal India Limited). PLSL was formerly the NCE R&D division of NPIL. PLSL has state-of-the-art R&D laboratories built over 200,000 square-feet of space in Mumbai, India and over 300 scientists engaged in drug discovery and development.

Post: Senior Research Scientist

Job Description:
Key Roles/Responsibilities:

Perform Metabolomics studies to generate of metabolite profiling (endogenous metabolites) data from cell culture and biofluid samples using high resolution liquid chromatography-mass spectrometry (HR-LC-MS). Carry out complete activities in the Metabolomics pipeline, from sample preparation, MS-based metabolite analyses to data treatment and statistical analysis
Set-up MS based shotgun and comprehensive proteomics workflows to perform protein fractionation, 2D gel analysis, in-gel and in-solution sample preparation, label-free quantification, algorithm based database search and protein/peptide ID. Further, integration of proteomic data with metabolomic and pharmacological information to aid drug target discovery.
Maintain and operate MALDI, UPLC and nanoLC coupled with high resolution mass spec (HRMS) instrument such as Orbitrap and give training to other team members
To utilize and further develop LC-MS/MS-based methods for both non-targeted and targeted metabolomics and drug metabolism ID (Met-ID) in biological matrices (in vitro matrices, cells, tissues, tumors, plasma and urine). Preparation of LC-MS method development reports, SOPs and protocol for qualitative and quantitative metabolite and protein analysis.
Tracer based metabolomics studies using HRMS tools such as isotopomer analysis, 13C labeled tracing of metabolism and flux analysis with survey, neutral-loss and precursor ion scans along with fragmentation tree generation, spectral interpretation and library search.
Structural identification and characterization of unknown metabolites using HRMS and MS/MS data
To coordinate with other research groups to plan and execute in vitro, cell culture, and animal based proteomic and metabolomic experiments
Generation and management of sample bank, metabolite and protein standards, spectral libraries and effectively integrate them with open-source databases
Perform large scale MS data processing and mining using software such as XCMS, Mzmine, MetAlign, R, Matlab, Mascot, Expressionist, SEQUEST and MetaboAnalyst.
Perform multivariate statistical data analysis using PCA, PLS, PLS-DA, OPLS methods and carry out network-based modeling of metabolomic and proteomic data sets

Additional Information:
Experience: 0-5 year for masters/ 0-3 years for Ph.D
Location: Goregaon, Mumbai
Industry Type: Pharma/ Biotech/Clinical Research
Functional Area: R&D
Education: M.Sc/M.Pharm/Ph.D